(8 references)-
- 抗原 See all IL7R 抗体
- IL7R (Interleukin 7 Receptor (IL7R))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This IL7R antibody is conjugated to Biotin
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应用范围
- Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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The B12-1 monoclonal antibody reacts with CD127, the 65-75- kDa type I transmembrane protein IL- 7Rα. The high affinity IL-7 receptor complex is composed of at least two transmembrane proteins, IL-7Rα and CD132, the common γ chain. CD127 has some sequence homology to the cytokine receptor superfamily (also known as the hematopoietin receptor superfamily). Interaction between IL-7 and its receptor is important for the proliferation of pre-B lymphocytes and can also trigger proliferation of CD4-CD8- immature thymocytes, as well as mature T cells in the periphery. Mice lacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious non-lymphoid abnormalities.5 IL-7Rα is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, on the earliest thymocyte progenitors and CD4-CD8- double-negative and CD4+ and CD8+ singlepositive thymocytes, and on most peripheral T lymphocytes. Intestinal intraepithelial lymphocytes with low-density γδ TCR upregulate CD127 expression in response to IL-2, which may be secreted by neighboring αβ TCR-bearing T cells. Expression of CD127 in the bone marrow and spleen. C57BL/6 bone marrow leukocytes (Left panels) and splenocytes (Right panels) were stained with either biotinylated rat IgG2a, κ isotype control mAb R35- 95 (Cat. no. 553928, Top panels) or biotinylated B12-1 antibody (Bottom panels) and either FITC-conjugated RA3-6B2 anti-mouse CD45R/B220, Cat. no. 553087/553088, Left panels) or FITC- conjugated 145-2C11 mAb (anti-mouse CD3e, Cat. no. 553061/553062, Right panels), followed by Streptavidin-PE (Cat. no. 554061, all panels). Flow cytometry was performed on a FACScan .
BD Pharmingen™ Biotin Rat Anti-Mouse CD127 - Biotin - Clone B12-1 - Isotype Rat IgG2a, λ - Reactivity Ms - 0.5 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Mouse CD127-human IgG fusion protein
- 克隆位点
- B12
- 亚型
- IgG2a lambda
- Top Product
- Discover our top product IL7R Primary Antibody
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anti-Interleukin 7 Receptor (IL7R) (AA 173-260) antibody
IL7R 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 Receptor (IL7R) (AA 21-239) antibodyIL7R 适用: 人 FACS, WB, ELISA, IHC, ICC, Neut 宿主: 小鼠 Monoclonal 3F5D9 unconjugated
anti-Interleukin 7 Receptor (IL7R) (AA 21-239) antibodyIL7R 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 Receptor (IL7R) (AA 21-236) antibodyIL7R 适用: 人 WB, ELISA, Coat 宿主: 小鼠 Monoclonal IL7R-2751 unconjugated
anti-Interleukin 7 Receptor (IL7R) (N-Term) antibodyIL7R 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 Receptor (IL7R) (pTyr449) antibodyIL7R 适用: 小鼠 WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 Receptor (IL7R) (pTyr449) antibodyIL7R 适用: 人 FACS, WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 Receptor (IL7R) antibody (APC)IL7R 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal A7R34 APC
anti-Interleukin 7 Receptor (IL7R) antibodyIL7R 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 7 Receptor (IL7R) (Ser552) antibodyIL7R 适用: 人, 小鼠, 猴 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Role of interleukin-7 in T-cell development from hematopoietic stem cells." in: Immunological reviews, Vol. 165, pp. 13-28, (1999) (PubMed).
: "Interleukin 2 (IL-2) and interleukin 7 (IL-7) reciprocally induce IL-7 and IL-2 receptors on gamma delta T-cell receptor-positive intraepithelial lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 8, pp. 3613-8, (1996) (PubMed).
: "Interleukin-2 receptor gamma chain: a functional component of the interleukin-7 receptor." in: Science (New York, N.Y.), Vol. 262, Issue 5141, pp. 1877-80, (1994) (PubMed).
: "Early lymphocyte expansion is severely impaired in interleukin 7 receptor-deficient mice." in: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1955-60, (1994) (PubMed).
: "Distinctive growth requirements and gene expression patterns distinguish progenitor B cells from pre-B cells." in: The Journal of experimental medicine, Vol. 177, Issue 4, pp. 915-23, (1993) (PubMed).
: "Expression and function of the interleukin 7 receptor in murine lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 19, pp. 9125-9, (1993) (PubMed).
: "Status of kappa L chain gene rearrangements and c-kit and IL-7 receptor expression in stromal cell-dependent pre-B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 6, pp. 1973-9, (1992) (PubMed).
: "Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily." in: Cell, Vol. 60, Issue 6, pp. 941-51, (1990) (PubMed).
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: "Role of interleukin-7 in T-cell development from hematopoietic stem cells." in: Immunological reviews, Vol. 165, pp. 13-28, (1999) (PubMed).
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- 抗原
- IL7R (Interleukin 7 Receptor (IL7R))
- 别名
- CD127 (IL7R 产品)
- 别名
- IL7R antibody, CD127 antibody, IL-7Ralpha antibody, CDW127 antibody, IL-7R-alpha antibody, IL7RA antibody, ILRA antibody, IL-7RA antibody, interleukin 7 receptor antibody, IL7R antibody, Il7r antibody
- 背景
- Synonyms: IL-7 Receptor α chain
- 途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size
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