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- 抗原 See all CD11b (ITGAM) 抗体
- CD11b (ITGAM) (Integrin alpha M (ITGAM))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This CD11b antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 品牌
- BD Pharmingen™
- 产品特性
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The M1/70 monoclonal antibody specifically binds to CD11b, also known as Integrin alpha M (Itgam or αM). CD11b is a 170- kDa type 1 transmembrane glycoprotein and belongs to the Integrin alpha chain family. CD11b serves as the alpha chain of the heterodimeric Mac-1 integrin (CD11b/CD18, αMβ2), also known as complement receptor 3 (CR3). Mac-1 mediates adhesion to ICAM-1 (CD54), ICAM-2 (CD102), fibrinogen and binding to C3bi. Mac-1 is expressed at varying levels on granulocytes, macrophages, myeloid-derived dendritic cells, natural killer cells, microglia, and B-1 B lymphocytes. Mac-1 expression is rapidly up-regulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface. The M1/70 antibody reportedly blocks cell adherence and C3bi binding but does not block cell-mediated lysis. Cross-reaction of the M1/70 antibody with CD11b expressed on human monocytes, polymorphonuclear leukocytes, and NK cells has been reported. Immunohistochemical staining of CD11b positive cells. An acetone-fixed, frozen section of normal mouse spleen was stained with the Purified Rat Anti-Mouse CD11b antibody. CD11b positive cells can be identified by the intense brown labeling of cell surface membranes (20X magnification).
BD Pharmingen™ Purified Rat Anti-Mouse CD11b - Purified - Clone M1/70 - Isotype Rat IgG2b, κ - Reactivity Ms - 1 mL - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Mouse Splenic Cells
- 克隆位点
- M1-70
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product ITGAM Primary Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 125 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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Wolfram syndrome 1 (Wfs1) gene expression in the normal mouse visual system." in: The Journal of comparative neurology, Vol. 510, Issue 1, pp. 1-23, (2008) (PubMed).
: "Markers of mouse macrophage development detected by monoclonal antibodies." in: Journal of immunological methods, Vol. 174, Issue 1-2, pp. 5-19, (1994) (PubMed).
: "The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells." in: The Journal of experimental medicine, Vol. 176, Issue 1, pp. 47-58, (1992) (PubMed).
: "Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets." in: The American journal of pathology, Vol. 111, Issue 1, pp. 112-24, (1983) (PubMed).
: "Mac-1: a macrophage differentiation antigen identified by monoclonal antibody." in: European journal of immunology, Vol. 9, Issue 4, pp. 301-6, (1979) (PubMed).
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Wolfram syndrome 1 (Wfs1) gene expression in the normal mouse visual system." in: The Journal of comparative neurology, Vol. 510, Issue 1, pp. 1-23, (2008) (PubMed).
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- 抗原
- CD11b (ITGAM) (Integrin alpha M (ITGAM))
- 别名
- CD11b (ITGAM 产品)
- 别名
- CD11b/CD18 antibody, CR3 antibody, CR3A antibody, Cd11b antibody, F730045J24Rik antibody, Ly-40 antibody, MAC1 antibody, Mac-1 antibody, Mac-1a antibody, CD11B antibody, MAC-1 antibody, MAC1A antibody, MO1A antibody, SLEB6 antibody, C3bi antibody, integrin subunit alpha M antibody, integrin alpha M antibody, integrin, alpha M (complement component 3 receptor 3 subunit) antibody, integrin alpha-M antibody, ITGAM antibody, Itgam antibody, LOC100351865 antibody
- 背景
- Synonyms: Itgam, Integrin alpha-M, Ly-40, Mac-1a, Mac-1 alpha, CR3A, CR-3 alpha chain
- 途径
- Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
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