Histone 3 抗体 (H3K4me3)
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- 抗原 See all Histone 3 (H3) 抗体
- Histone 3 (H3)
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抗原表位
- H3K4me3
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适用
- 人, Saccharomyces cerevisiae
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Histone 3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
- 纯化方法
- Protein A Chromatography
- 免疫原
- This Histone H3 trimethylLys4 antibody was raised against a peptide including trimethyllysine 4 of histone H3.
- 亚型
- IgG
- Top Product
- Discover our top product H3 Primary Antibody
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- 应用备注
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Recommended starting concentrations are
ChIP: 3 µg per ChIP
ChIP-Seq: 4 µg each
ICC/IF: 1 - 2 µg/mL dilution
WB: 2 - 4 µg/mL dilution
CUT&RUN: 1:100
Optimal working dilution should be determined by the investigator. - 限制
- 仅限研究用
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- 浓度
- 1 μg/μL
- 缓冲液
- Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- 储存条件
- -20 °C
- 储存方法
- Antibodies in solution can be stored at -20 °C for 2 years.
- 有效期
- 6 months
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H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).
: "Reduced expression of SET7/9, a histone mono-methyltransferase, is associated with gastric cancer progression." in: Oncotarget, Vol. 7, Issue 4, pp. 3966-83, (2016) (PubMed).
: "Alterations in nuclear structure promote lupus autoimmunity in a mouse model." in: Disease models & mechanisms, Vol. 9, Issue 8, pp. 885-97, (2016) (PubMed).
: "The long noncoding RNA HOTAIR has tissue and cell type-dependent effects on HOX gene expression and phenotype of urothelial cancer cells." in: Molecular cancer, Vol. 14, pp. 108, (2015) (PubMed).
: "A balancing act: heterochromatin protein 1a and the Polycomb group coordinate their levels to silence chromatin in Drosophila." in: Epigenetics & chromatin, Vol. 8, pp. 17, (2015) (PubMed).
: "Sex Specification and Heterogeneity of Primordial Germ Cells in Mice." in: PLoS ONE, Vol. 10, Issue 12, pp. e0144836, (2015) (PubMed).
: "Loss of histone H3 methylation at lysine 4 triggers apoptosis in Saccharomyces cerevisiae." in: PLoS genetics, Vol. 10, Issue 1, pp. e1004095, (2014) (PubMed).
: "The 3D tissue microenvironment modulates DNA methylation and E-cadherin expression in squamous cell carcinoma." in: Epigenetics, Vol. 7, Issue 1, pp. 34-46, (2012) (PubMed).
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H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).
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- 抗原
- Histone 3 (H3)
- 别名
- Histone H3 (H3 产品)
- 别名
- H-3 antibody, histocompatibility 3 antibody, H3 antibody
- 分子量
- 17 kDa
- 基因ID
- 3020
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