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Histone 3 抗体 (H3K4me3)

H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, DB, ICC, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN2668474
发货至: 中国
  • 抗原 See all Histone 3 (H3) 抗体
    Histone 3 (H3)
    抗原表位
    • 59
    • 47
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    • 37
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    H3K4me3
    适用
    • 1399
    • 941
    • 861
    • 59
    • 42
    • 41
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    • 35
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    人, Saccharomyces cerevisiae
    宿主
    • 1217
    • 243
    • 8
    • 4
    克隆类型
    • 1120
    • 351
    • 1
    多克隆
    标记
    • 821
    • 81
    • 63
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    • 37
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    • 23
    • 17
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    • 17
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    • 2
    • 1
    This Histone 3 antibody is un-conjugated
    应用范围
    • 1058
    • 411
    • 378
    • 313
    • 251
    • 188
    • 181
    • 179
    • 163
    • 160
    • 141
    • 126
    • 119
    • 48
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    • 15
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    • 1
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    • 1
    Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
    纯化方法
    Protein A Chromatography
    免疫原
    This Histone H3 trimethylLys4 antibody was raised against a peptide including trimethyllysine 4 of histone H3.
    亚型
    IgG
    Top Product
    Discover our top product H3 Primary Antibody
  • 应用备注
    Recommended starting concentrations are
    ChIP: 3 µg per ChIP
    ChIP-Seq: 4 µg each
    ICC/IF: 1 - 2 µg/mL dilution
    WB: 2 - 4 µg/mL dilution
    CUT&RUN: 1:100
    Optimal working dilution should be determined by the investigator.
    限制
    仅限研究用
  • 浓度
    1 μg/μL
    缓冲液
    Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项

    Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

    储存条件
    -20 °C
    储存方法
    Antibodies in solution can be stored at -20 °C for 2 years.
    有效期
    6 months
  • Zhang, Li, Rezaeian, Xu, Chou, Jin, Han, Pan, Wang, Long, Zhang, Huang, Tsai, Tsai, Logothetis, Lin: "H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).

    Akiyama, Koda, Byeon, Shimada, Nishikawaji, Sakamoto, Chen, Kojima, Kawano, Eishi, Deng, Kim, Zhu, Yuasa, Tanaka: "Reduced expression of SET7/9, a histone mono-methyltransferase, is associated with gastric cancer progression." in: Oncotarget, Vol. 7, Issue 4, pp. 3966-83, (2016) (PubMed).

    Singh, Johnstone, Martin, Tempera, Kaplan, Denny: "Alterations in nuclear structure promote lupus autoimmunity in a mouse model." in: Disease models & mechanisms, Vol. 9, Issue 8, pp. 885-97, (2016) (PubMed).

    Heubach, Monsior, Deenen, Niegisch, Szarvas, Niedworok, Schulz, Hoffmann: "The long noncoding RNA HOTAIR has tissue and cell type-dependent effects on HOX gene expression and phenotype of urothelial cancer cells." in: Molecular cancer, Vol. 14, pp. 108, (2015) (PubMed).

    Cabrera, Olcese, Horabin: "A balancing act: heterochromatin protein 1a and the Polycomb group coordinate their levels to silence chromatin in Drosophila." in: Epigenetics & chromatin, Vol. 8, pp. 17, (2015) (PubMed).

    Sakashita, Kawabata, Jincho, Tajima, Kumamoto, Kobayashi, Matsui, Kono: "Sex Specification and Heterogeneity of Primordial Germ Cells in Mice." in: PLoS ONE, Vol. 10, Issue 12, pp. e0144836, (2015) (PubMed).

    Walter, Matter, Fahrenkrog: "Loss of histone H3 methylation at lysine 4 triggers apoptosis in Saccharomyces cerevisiae." in: PLoS genetics, Vol. 10, Issue 1, pp. e1004095, (2014) (PubMed).

    DesRochers, Shamis, Alt-Holland, Kudo, Takata, Wang, Jackson-Grusby, Garlick: "The 3D tissue microenvironment modulates DNA methylation and E-cadherin expression in squamous cell carcinoma." in: Epigenetics, Vol. 7, Issue 1, pp. 34-46, (2012) (PubMed).

  • 抗原
    Histone 3 (H3)
    别名
    Histone H3 (H3 产品)
    别名
    H-3 antibody, histocompatibility 3 antibody, H3 antibody
    分子量
    17 kDa
    基因ID
    3020
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