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Histone 3 抗体 (H3K9me3)

Name: Histone 3 抗体 (H3K9me3)
SKU: ABIN2668470
Availability: OutOfStock

Cited in 33+ publications. This 兔多克隆 anti-Histone 3 antibody specifically detects Histone 3 in WB, IF, ChIP, ICC, DB 和 ChIP-seq. The antibody is reactive with 人和 Schizosaccharomyces pombe samples.

Histone H3K9me3 antibody (pAb) tested by Western blot. (Histone 3 抗体 (H3K9me3))

Dot blot of Histone H3 trimethyl Lys9 antibody. (Histone 3 抗体 (H3K9me3))

Histone H3 trimethyl Lys9 antibody specificity tested by peptide array analysis. (Histone 3 抗体 (H3K9me3))

PAr

Histone H3 trimethyl Lys9 antibody tested by ChIP-chip. (Histone 3 抗体 (H3K9me3))

ChIP-seq

Histone H3 trimethyl Lys9 antibody tested by immunofluorescence. (Histone 3 抗体 (H3K9me3))

5 images

(33 references)

产品编号 ABIN2668470

发货至: 中国

Contact our Customer Service for availability and price in your country. Contact Info

Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Histone 3 抗体 (H3K9me3) (ABIN2668470)

抗原

See all Histone 3 (H3) 抗体

Histone 3 (H3) (Histone H3 (H3))

适用

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人, Schizosaccharomyces pombe

宿主

兔

克隆类型

多克隆

标记

This Histone 3 antibody is un-conjugated

应用范围

1330
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Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Discover our top product H3 Primary Antibody

抗原表位
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10
H3K9me3

纯化方法

None

免疫原

This Histone H3 trimethylLys9 antibody was raised against a peptide including trimethyllysine 9 of histone H3.
anti-Histone H3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 适用: 人, 小鼠 WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 小鼠 Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27me) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (C-Term) antibody
H3 适用: 人 WB, IHC, ChIP, IP 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3R17me) antibody
H3 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

应用备注

Recommended antibody dilutions for different applications:
ChIP: 2 - 10 µl per ChIP
ChIP-Seq: 10 µl each
ICC/IF: 1:500 - 1:1,000 dilution
WB: 1:1,000 - 1:5,000 dilution
CUT&Tag: 1:50 dilution (1 µl per 50 µl reaction)
Optimal working dilution should be determined by the investigator.

限制

仅限研究用
状态

Liquid

缓冲液

Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

储存条件

-20 °C

储存方法

Antibodies in solution can be stored at -20 °C for 2 years.

有效期

6 months
Egan, Yuan, Craske, Labhart, Guler, Arnott, Maile, Busby, Henry, Kelly, Tindell, Jhunjhunwala, Zhao, Hatton, Bryant, Classon, Trojer: "An Alternative Approach to ChIP-Seq Normalization Enables Detection of Genome-Wide Changes in Histone H3 Lysine 27 Trimethylation upon EZH2 Inhibition." in: PLoS ONE, Vol. 11, Issue 11, pp. e0166438, (2016) (PubMed).

Thompson, Dulberg, Moon, Foster, Chen, Karimi, Lorincz: "hnRNP K coordinates transcriptional silencing by SETDB1 in embryonic stem cells." in: PLoS genetics, Vol. 11, Issue 1, pp. e1004933, (2015) (PubMed).

Postberg, Kanders, Forcob, Willems, Orth, Hensel, Weil, Wirth, Jenke: "CpG signalling, H2A.Z/H3 acetylation and microRNA-mediated deferred self-attenuation orchestrate foetal NOS3 expression." in: Clinical epigenetics, Vol. 7, Issue 1, pp. 9, (2015) (PubMed).

Cabrera, Olcese, Horabin: "A balancing act: heterochromatin protein 1a and the Polycomb group coordinate their levels to silence chromatin in Drosophila." in: Epigenetics & chromatin, Vol. 8, pp. 17, (2015) (PubMed).

Soyer, Möller, Schotanus, Connolly, Galazka, Freitag, Stukenbrock: "Chromatin analyses of Zymoseptoria tritici: Methods for chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq)." in: Fungal genetics and biology : FG & B, Vol. 79, pp. 63-70, (2015) (PubMed).

Amatori, Ballarini, Faversani, Belloni, Fusar, Bosari, Pelicci, Minucci, Fanelli: "PAT-ChIP coupled with laser microdissection allows the study of chromatin in selected cell populations from paraffin-embedded patient samples." in: Epigenetics & chromatin, Vol. 7, pp. 18, (2014) (PubMed).

Soyer, El Ghalid, Glaser, Ollivier, Linglin, Grandaubert, Balesdent, Connolly, Freitag, Rouxel, Fudal: "Epigenetic control of effector gene expression in the plant pathogenic fungus Leptosphaeria maculans." in: PLoS genetics, Vol. 10, Issue 3, pp. e1004227, (2014) (PubMed).

Minkovsky, Sahakyan, Rankin-Gee, Bonora, Patel, Plath: "The Mbd1-Atf7ip-Setdb1 pathway contributes to the maintenance of X chromosome inactivation." in: Epigenetics & chromatin, Vol. 7, pp. 12, (2014) (PubMed).

Rangasamy: "Distinctive patterns of epigenetic marks are associated with promoter regions of mouse LINE-1 and LTR retrotransposons." in: Mobile DNA, Vol. 4, Issue 1, pp. 27, (2013) (PubMed).

Maksakova, Thompson, Goyal, Jones, Singh, Karimi, Lorincz: "Distinct roles of KAP1, HP1 and G9a/GLP in silencing of the two-cell-specific retrotransposon MERVL in mouse ES cells." in: Epigenetics & chromatin, Vol. 6, Issue 1, pp. 15, (2013) (PubMed).

Thijssen, Tobi, Balog, Schouten, Kremer, El Bouazzaoui, Henneman, Putter, Eline Slagboom, Heijmans, van der Maarel: "Chromatin remodeling of human subtelomeres and TERRA promoters upon cellular senescence: commonalities and differences between chromosomes." in: Epigenetics, Vol. 8, Issue 5, pp. 512-21, (2013) (PubMed).

Karimi-Aghcheh, Bok, Phatale, Smith, Baker, Lichius, Omann, Zeilinger, Seiboth, Rhee, Keller, Freitag, Kubicek: "Functional analyses of Trichoderma reesei LAE1 reveal conserved and contrasting roles of this regulator." in: G3 (Bethesda, Md.), Vol. 3, Issue 2, pp. 369-78, (2013) (PubMed).

Connolly, Smith, Freitag: "The Fusarium graminearum histone H3 K27 methyltransferase KMT6 regulates development and expression of secondary metabolite gene clusters." in: PLoS genetics, Vol. 9, Issue 10, pp. e1003916, (2013) (PubMed).

Murata, Narita, Sugimoto, Kawashima, Kanda, Tsurumi: "Contribution of myocyte enhancer factor 2 family transcription factors to BZLF1 expression in Epstein-Barr virus reactivation from latency." in: Journal of virology, Vol. 87, Issue 18, pp. 10148-62, (2013) (PubMed).

Wiemann, Sieber, von Bargen, Studt, Niehaus, Espino, Huß, Michielse, Albermann, Wagner, Bergner, Connolly, Fischer, Reuter, Kleigrewe, Bald, Wingfield, Ophir, Freeman, Hippler, Smith, Brown, Proctor et al.: "Deciphering the cryptic genome: genome-wide analyses of the rice pathogen Fusarium fujikuroi reveal complex regulation of secondary metabolism and novel metabolites. ..." in: PLoS pathogens, Vol. 9, Issue 6, pp. e1003475, (2013) (PubMed).

Jamieson, Rountree, Lewis, Stajich, Selker: "Regional control of histone H3 lysine 27 methylation in Neurospora." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 15, pp. 6027-32, (2013) (PubMed).

Murphy, Cipriany, Wallin, Ju, Szeto, Hagarman, Benitez, Craighead, Soloway: "Single-molecule analysis of combinatorial epigenomic states in normal and tumor cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 19, pp. 7772-7, (2013) (PubMed).

Woellmer, Arteaga-Salas, Hammerschmidt: "BZLF1 governs CpG-methylated chromatin of Epstein-Barr Virus reversing epigenetic repression." in: PLoS pathogens, Vol. 8, Issue 9, pp. e1002902, (2012) (PubMed).

Seiboth, Karimi, Phatale, Linke, Hartl, Sauer, Smith, Baker, Freitag, Kubicek: "The putative protein methyltransferase LAE1 controls cellulase gene expression in Trichoderma reesei." in: Molecular microbiology, Vol. 84, Issue 6, pp. 1150-64, (2012) (PubMed).

Duncan, Barwick, Jin, Rago, Kapoor-Vazirani, Powell, Chi, Bigner, Vertino, Yan: "A heterozygous IDH1R132H/WT mutation induces genome-wide alterations in DNA methylation." in: Genome research, Vol. 22, Issue 12, pp. 2339-55, (2012) (PubMed).

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抗原

Histone 3 (H3) (Histone H3 (H3))

别名

Histone H3

分子量

17 kDa

基因ID

3020