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Histone 3 抗体 (H3K9me3)

H3 适用: 人 WB, IF, ChIP, DB, ICC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN2668405
发货至: 中国
  • 抗原 See all Histone 3 (H3) 抗体
    Histone 3 (H3)
    抗原表位
    • 57
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    H3K9me3
    适用
    • 1379
    • 940
    • 861
    • 59
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    宿主
    • 1198
    • 242
    • 8
    • 4
    克隆类型
    • 1121
    • 330
    • 1
    多克隆
    标记
    • 810
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    • 34
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    This Histone 3 antibody is un-conjugated
    应用范围
    • 1048
    • 408
    • 374
    • 311
    • 249
    • 188
    • 176
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    • 163
    • 160
    • 137
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    • 118
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    Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC)
    纯化方法
    Protein A Chromatography
    免疫原
    This Histone H3 trimethylLys9 antibody was raised against a peptide including trimethyllysine 9 of histone H3.
    亚型
    IgG
    Top Product
    Discover our top product H3 Primary Antibody
  • 应用备注
    Recommended antibody dilutions for different applications:
    ChIP: 10 µg for each ChIP
    ICC/IF: 1 µg/mL dilution
    WB: 0.5 - 2 µg/mL dilution
    CUT&Tag: 1 µg for each 50 µL reaction
    Optimal working dilution should be determined by the investigator.
    限制
    仅限研究用
  • 浓度
    1 μg/μL
    缓冲液
    Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项

    Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

    储存条件
    -20 °C
    储存方法
    Antibodies in solution can be stored at -20 °C for 2 years.
    有效期
    6 months
  • Zhang, Li, Rezaeian, Xu, Chou, Jin, Han, Pan, Wang, Long, Zhang, Huang, Tsai, Tsai, Logothetis, Lin: "H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).

    Maison, Bailly, Quivy, Almouzni: "The methyltransferase Suv39h1 links the SUMO pathway to HP1α marking at pericentric heterochromatin." in: Nature communications, Vol. 7, pp. 12224, (2016) (PubMed).

    Quattrocelli, Swinnen, Giacomazzi, Camps, Barthélemy, Ceccarelli, Caluwé, Grosemans, Thorrez, Pelizzo, Muijtjens, Verfaillie, Blot, Janssens, Sampaolesi: "Mesodermal iPSC-derived progenitor cells functionally regenerate cardiac and skeletal muscle." in: The Journal of clinical investigation, Vol. 125, Issue 12, pp. 4463-82, (2015) (PubMed).

    Lu, Venneti, Akalin, Fang, Ward, Dematteo, Intlekofer, Chen, Ye, Hameed, Nafa, Agaram, Cross, Khanin, Mason, Healey, Lowe, Schwartz, Melnick, Thompson: "Induction of sarcomas by mutant IDH2." in: Genes & development, Vol. 27, Issue 18, pp. 1986-98, (2013) (PubMed).

    Tennakoon, Wang, Coarfa, Cooney, Gunaratne: "Chromatin changes in dicer-deficient mouse embryonic stem cells in response to retinoic acid induced differentiation." in: PLoS ONE, Vol. 8, Issue 9, pp. e74556, (2013) (PubMed).

    Chen, Liu, Lu, Cross, Morris, Shroff, Ward, Bradner, Thompson, Lowe: "Cancer-associated IDH2 mutants drive an acute myeloid leukemia that is susceptible to Brd4 inhibition." in: Genes & development, Vol. 27, Issue 18, pp. 1974-85, (2013) (PubMed).

    Rothbart, Krajewski, Nady, Tempel, Xue, Badeaux, Barsyte-Lovejoy, Martinez, Bedford, Fuchs, Arrowsmith, Strahl: "Association of UHRF1 with methylated H3K9 directs the maintenance of DNA methylation." in: Nature structural & molecular biology, Vol. 19, Issue 11, pp. 1155-60, (2012) (PubMed).

  • 抗原
    Histone 3 (H3)
    别名
    Histone H3 (H3 产品)
    别名
    H-3 antibody, histocompatibility 3 antibody, H3 antibody
    分子量
    17 kDa
    基因ID
    3020
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