GAPDH 抗体
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北京 101111
Quick Overview for GAPDH 抗体 (ABIN263740)
抗原
See all GAPDH 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognizes GAPDH monomer (36 kDa) and the dimer form. Does not react with the tetrameric form.
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产品特性
- Synonyms: GAPD, CDABP0047, Loading control, Glyceraldehyde-3-Phosphate Dehydrogenase
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纯化方法
- Protein A Chromatography.
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免疫原
- Rabbit muscle GAPDH. Hybridization of Sp2/0 myeloma cells with spleen cells from BALB/cmice.
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亚型
- IgG1
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应用备注
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ELISA. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 4.1 mg/mL (Sigma protein assay kit)
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缓冲液
- PBS, pH 7.4 containing 0,09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
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Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch. -
有效期
- 12 months
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- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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别名
- GAPDH
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背景
- Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed in almost all tissues at high levels, therefore becoming the marker of choice when a loading control in Western blotting is required. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase
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基因ID
- 2597
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UniProt
- P04406
抗原
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