ATP6AP1 抗体
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- 抗原 See all ATP6AP1 抗体
- ATP6AP1 (ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1))
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适用
- Arabidopsis thaliana, Chlamydomonas, Soybean
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ATP6AP1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunolocalization (IL)
- 交叉反应
- Soybean (Glycine max)
- 交叉反应 (详细)
- No cross-reactivity with: Aspergillus niger
- 产品特性
- Expected / apparent Molecular Weight of the Antigene: 95 kDa (Arabidopsis thaliana)
- 纯化方法
- serum
- 免疫原
- KLH-conjugated synthetic peptide derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (At2g18960) and ATPase 2,3,4,6,7,8,9 of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)
- Top Product
- Discover our top product ATP6AP1 Primary Antibody
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- 应用备注
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Recommended Dilution: 1 : 1000 - 1 : 5000 with standard ECL OR 1 : 10 000 with ECL-Advance, enhanced chemiluminescence (WB), 1 : 600 - 1 : 1000 (IF).
Cellular [compartment marker] for plasma membrane - 说明
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for additional Western blot detection image please refer to the article below.VERY IMPORTANT: please, do not heat up your samples over 70°C as this might cause H+ATPase to precipitate and there will be no signal on your western blot.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 200 µL of sterile water.
- 缓冲液
- PBS pH 7.4
- 注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - 储存条件
- -20 °C
- 储存方法
- Do not store this antibody at 4 °C.
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The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." in: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).
: "Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family." in: Journal of experimental botany, Vol. 64, Issue 14, pp. 4375-87, (2013) (PubMed).
: "Plasma membrane-associated malate dehydrogenase of maize (Zea mays L.) roots: Native versus recombinant protein." in: Journal of proteomics, Vol. 80C, pp. 66-77, (2013) (PubMed).
: "Plasma membrane H(+) -ATPase gene expression, protein level and activity in growing and non-growing regions of barley (Hordeum vulgare) leaves." in: Physiologia plantarum, Vol. 144, Issue 4, pp. 382-93, (2012) (PubMed).
: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).
: "Analysis of the Arabidopsis Cytosolic Proteome Highlights Subcellular Partitioning of Central Plant Metabolism." in: Journal of proteome research, (2010) (PubMed).
: "Nitric oxide enhances salt secretion and Na(+) sequestration in a mangrove plant, Avicennia marina, through increasing the expression of H(+)-ATPase and Na(+)/H(+) antiporter under high salinity." in: Tree physiology, Vol. 30, Issue 12, pp. 1570-85, (2010) (PubMed).
: "Two Chlamydomonas CTR copper transporters with a novel cys-met motif are localized to the plasma membrane and function in copper assimilation." in: The Plant cell, Vol. 21, Issue 3, pp. 928-43, (2009) (PubMed).
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The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." in: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).
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- 抗原
- ATP6AP1 (ATPase, H+ Transporting, Lysosomal Accessory Protein 1 (ATP6AP1))
- 别名
- H+ATPase (ATP6AP1 产品)
- 别名
- 16A antibody, ATP6IP1 antibody, ATP6S1 antibody, Ac45 antibody, CF2 antibody, VATPS1 antibody, XAP-3 antibody, XAP3 antibody, AC45 antibody, AI316502 antibody, AW108110 antibody, Atp6ip1 antibody, Atp6s1 antibody, C7-1 antibody, mFLJ00383 antibody, H+-ATPase antibody, ATPase H+ transporting accessory protein 1 antibody, ATPase, H+ transporting, lysosomal accessory protein 1 antibody, plasma membrane ATPase 4 antibody, ATP6AP1 antibody, Atp6ap1 antibody, LOC547525 antibody
- 背景
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AGI Code: At2g18960
The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves. - 分子量
- 95 kDa (Arabidopsis thaliana)
- UniProt
- P20649
- 途径
- Proton Transport, SARS-CoV-2 Protein Interactome
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