SLC12A3 抗体 (AA 74-95) (APC)
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- 抗原 See all SLC12A3 抗体
- SLC12A3 (Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3))
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抗原表位
- AA 74-95
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SLC12A3 antibody is conjugated to APC
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoelectron Microscopy (IEM)
- 特异性
- Detects ~160 kDa.
- 交叉反应
- 犬, 人, 小鼠, 大鼠
- 纯化方法
- Affinity Purified
- 免疫原
- Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal
- Top Product
- Discover our top product SLC12A3 Primary Antibody
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- 应用备注
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- WB (1:1000)
- IHC (1:200)
- optimal dilutions for assays should be determined by the user.
- 说明
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1 μg/ml of ABIN2486291 was sufficient for detection of NCC3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- SLC12A3 (Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3))
- 别名
- NCC (SLC12A3 产品)
- 别名
- SLC12A3 antibody, slc12a3 antibody, DKFZp469N2315 antibody, NCC antibody, NCCT antibody, TSC antibody, AI035291 antibody, solute carrier family 12 member 3 antibody, solute carrier family 12 (sodium/chloride transporter), member 3 antibody, solute carrier family 12, member 3 antibody, SLC12A3 antibody, slc12a3.2 antibody, Slc12a3 antibody
- 背景
- NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).
- 基因ID
- 54300, 6559
- NCBI登录号
- NP_062218, NP_000330
- UniProt
- P55018, P55017
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