TRC8 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
TRC8 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of TRC8.
RNF139
适用: 小鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 680
应用备注
ELISA: Antibody detection limit dilution 1:16000. Experiments gave bands at approximately 150 kDa and 50 kDa in human skeletal Mucle lysate after 0.5 μg/mL antibody staining. This protein has a calculated MW of 76.0 kDa according to NP_009149.2.The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-1.5 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.