Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
纯化方法
WNT3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
WNT3 antibody was raised against a 15 amino acid synthetic peptide near the internal region of WNT3.
ELISA: Antibody detection limit dilution 1:2000. Western Blot: Approximately 38 kDa band observed in human brain lysates (calculated MW of 39.6 kDa according to NP_110380.1). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human breast shows strong fibrous staining in myoepithelial cells. Recommended concentration, 3-5 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
WNT3
(Wingless-Type MMTV Integration Site Family, Member 3 (WNT3))