ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
特异性
This antibody is expected to recognize isoform 1 (NP_000359.1) only.
纯化方法
TSC1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
TSC1 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of TSC1.
ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 150 kDa band observed in human brain lysates (calculated MW of 130 kDa according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet. 2000 Jul 22,9 (12) :1721-7, PMID: 10915759). In transfected HEK293 transiently expressing human TSC1 a band of approximately 120 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.