This antibody is expected to recognize both human isoforms of this protein.
纯化方法
TRIM35 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
TRIM35 antibody was raised against a 13 amino acid synthetic peptide near the N-Terminus of TRIM35.
ELISA: Antibody detection limit dilution >1:32000. Western Blot: Approximately 25-30 kDa band observed in human testis and human brain lysates at 1 ?, this band was successfully blocked by incubation with the immunizing peptide. Please note that the band we observe is different from the predicted sizes of approximately 57 kDa and 24 kDa according to NP_055881and NP_741983. Approximately 50 kDa band observed in K562 lysate (calculated MW of 56.5 kDa according to NP_741983.2). Recommended concentration: 1-3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.