All reported variants (NP_000911.2, NP_001035806.1, NP_071504.2) represent identical protein.
纯化方法
Pyruvate carboxylase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
Pyruvate carboxylase antibody was raised against a 14 amino acid synthetic peptide near the internal region of Pyruvate carboxylase.
PC
适用: Saccharomyces cerevisiae
ELISA
宿主: 兔
Polyclonal
unconjugated
应用备注
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 150 kDa band observed in mouse liver lysates (calculated MW of 130 kDa according to NP_000911.2, NP_001035806.1and NP_071504.2). Recommended concentration: 0.03-0.1 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.