This antibody is not excpected to cross-react with PITX1 and PITX2.
纯化方法
PITX3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
PITX3 antibody was raised against a 12 amino acid synthetic peptide near the internal region of PITX3.
ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 40 kDa band observed in human brain (cerebellum and cerebral cortex) lysates (calculated MW of 31.8 kDa according to NP_005020.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Coulon et al, J Biol Chem. 2007 Nov 9,282 (45) :33192-200., PMID: 17848564). Recommended concentration: 0.3-1 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.