This antibody is expected to recognize all reported isoforms (NP_002591.2, NP_001032418.1, NP_001032416.1 and NP_001032417.1).
纯化方法
Phosphodiesterase 4B antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
Phosphodiesterase 4B antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of Phosphodiesterase 4B.
ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 85 kDa band observed in mouse brain lysates (calculated MW of 83.3 kDa according to human NP_002591.2 and 82.1 kDa according to mouse NP_062814.2). In transfected HEK293 transiently expressing human PDE4B a band of approximately 85 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.0 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.