This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2.
纯化方法
PDE5A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
PDE5A antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of PDE5A.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 100 kDa band observed in human lung lysates (calculated MW of 100 kDa according to isoform 1, NP_001074.2). In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approximately 95 kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8 kDa according to isoform 2, NP_236914.2). Recommended concentration: 0.3-1 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.