This antibody is expected to recognize both reported isoforms (NP_000908.2 and NP_001017962.1).
纯化方法
P4HA1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
P4HA1 antibody was raised against a 13 amino acid synthetic peptide near the internal region of P4HA1.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 60 kDa band observed in lysates of cell lines HEK293, A431 and NIH3T3 (calculated MW of 61.0 kDa according to NP_000908.2 and NP_001017962.1). Recommended concentration: 0.1-0.1 μg/mL. an additional band of unknown identity was also consistently observed at 48 kDa . This band was successfully blocked by incubation with the immunizing peptide.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
P4HA1
(Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))