NEDD1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
NEDD1 antibody was raised against a 14 amino acid synthetic peptide near the internal region of NEDD1.
NEDD1
适用: 人
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 680
应用备注
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 75 kDa band observed in HeLa lysate (calculated MW of 72.0 kDa according to NP_690869.1). Recommended concentration: 1-3 μg/mL. an additional band was also consistently observed at 37 kDa . This band was successfully blocked by incubation with the immunizing peptide and likely represents the mature form.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.