Western Blotting (WB), Immunohistochemistry (IHC), ELISA
特异性
This antibody is expected to recognize both reported isoforms (NP_000622.2 and NP_038202.1).
纯化方法
NCF4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
NCF4 antibody was raised against an 11 amino acid synthetic peptide near the internal region of NCF4.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 38 kDa band observed in lysates of Daudi lysate (calculated MW of 39.0 kDa according to NP_000622.2 and 38.8 kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approximately 42 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human tonsil shows staining of the cytoplasm in some cells. Recommended concentration, 2-4 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.