Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
纯化方法
MEPCE antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
MEPCE antibody was raised against a 13 amino acid synthetic peptide near the internal region of MEPCE.
ELISA: Antibody detection limit dilution 1:2,000. Western Blot: Approximately 75 kDa band observed in rat testis lysates (calculated MW of 74.4 kDa according to human NP_062552.2 and 75.3 kDa according to rat XP_222048.4). In transfected HEK293 transiently expressing human MEPCE a band of approximately 75 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL. IHC: In paraffin embedded Human Testis shows staining of the spermatids. Recommended concentration, 5-10μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.