This antibody is expected to recognize all reported isoforms (NP_055879.1, NP_829881.1, NP_829882.1, NP_829883.1, NP_829884.1).
纯化方法
ELKS antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
ELKS antibody was raised against a 13 amino acid synthetic peptide near the N-Terminus of ELKS.
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 85 kDa band observed in human pancreas and testis lysates (calculated MW of 82 kDa according to NP_829882, isoform gamma). A second band of 37 kDa is detected. Both bands are successfully blocked by incubation with the immunizing peptide. No proteins of approximatelyimately 37 kDa were found after a BLASTP search in NCBI with the immunizing peptide as a probe. Recommended concentration: 0.1-0.3 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
ERC1
(ELKS/RAB6-Interacting/CAST Family Member 1 (ERC1))