Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
特异性
This antibody is expected to recognize both human isoforms, as represented by NP_004073 and NP_075408.
纯化方法
Dynactin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
Dynactin antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of Dynactin.
ELISA: Antibody detection limit dilution 1:128000. Western Blot: Approximately 150 kDa band observed in cell lline A549 and in human testis lysates (calculated MW of 142 kDa according to NP_004073.2). Recommended for use at 0.5-2 μg/mL. Immunohistochemistry: In paraffin embedded human cerebellum shows pixulate staining of cytoplasm in neuronal cells. In paraffin embedded human testis shows cytoplasm staining in cells of the seminiferous tubules. Recommended concentration, 2-4 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.