Dynactin 1 抗体 (C-Term)
(1 reference)-
- 抗原 See all Dynactin 1 (DCTN1) 抗体
- Dynactin 1 (DCTN1)
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Dynactin 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- Dynactin
- 序列
- QEQLHQLHSR LIS
- 特异性
- This antibody is expected to recognise all reported isoforms.
- 交叉反应
- 人, 小鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1.
- 亚型
- IgG
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anti-Dynactin 1 (DCTN1) (C-Term) antibody
DCTN1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dynactin 1 (DCTN1) (AA 1084-1278) antibodyDCTN1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dynactin 1 (DCTN1) (C-Term) antibodyDCTN1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dynactin 1 (DCTN1) (AA 945-1139) antibodyDCTN1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Dynactin 1 (DCTN1) (N-Term) antibodyDCTN1 适用: 人 WB, IP 宿主: 小鼠 Monoclonal unconjugated
anti-Dynactin 1 (DCTN1) antibodyDCTN1 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Dynactin 1 (DCTN1) (AA 1-198) antibodyDCTN1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E4-1C2 unconjugated
anti-Dynactin 1 (DCTN1) (AA 1216-1278) antibodyDCTN1 适用: 人 WB, IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dynactin 1 (DCTN1) (C-Term) antibodyDCTN1 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 6-7 μg/mL.
Western Blot: Approx. 150 kDa band observed in HeLa and MCF7 cell lysates, and in preliminary testing of HEK293 and Mouse Brain lysate (calculated MW of 142 kDa according to NP_004073.2). Recommended concentration 0.3-1 μg/mL. Primary incubation 1 hour at
Peptide ELISA: antibody detection limit dilution 1:16000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells and additionally the nucleus of U2OS cells.. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeL
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro." in: American journal of physiology. Heart and circulatory physiology, Vol. 296, Issue 3, pp. H806-14, (2009) (PubMed).
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: "Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro." in: American journal of physiology. Heart and circulatory physiology, Vol. 296, Issue 3, pp. H806-14, (2009) (PubMed).
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- 抗原
- Dynactin 1 (DCTN1)
- 别名
- DCTN1 (DCTN1 产品)
- 别名
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DAP-150 antibody, DP-150 antibody, P135 antibody, Chip150 antibody, DCTN1 antibody, DKFZp469C1813 antibody, AL022633 antibody, Glued antibody, p150 antibody, p150
antibody, CG9206 antibody, DYNA_DROME antibody, Dmel\\CG9206 antibody, FCP-D antibody, Glu antibody, P150 antibody, P150[Glued] antibody, p150/Glued antibody, p150/glued antibody, p150[Glued] antibody, p150[glued] antibody, t1 antibody, Dynactin-1 antibody, glued antibody, dynactin subunit 1 antibody, dynactin 1 antibody, Dynactin 1, p150 subunit antibody, dynactin subunit 1 L homeolog antibody, DCTN1 antibody, Dctn1 antibody, DCTN1-p150 antibody, dctn1.L antibody - 背景
- DCTN1, dynactin 1 (p150, glued homolog, Drosophila), P135, DP-150, DAP-150, p150-glued, p150, Glued (Drosophila) homolog, 150 kDa dynein-associated polypeptide, dynactin 1 (p150, Glued (Drosophila) homolog), HMN7B, OTTHUMP00000202491, dynactin 1, p150, Gl
- 基因ID
- 1639, 13191
- NCBI登录号
- NP_004073, NP_075408, NP_001128512, NP_001128513, NP_001177765, NP_001177766
- 途径
- M Phase, ER-Nucleus Signaling
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