Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
纯化方法
Duffy antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
Duffy antibody was raised against a 19 amino acid synthetic peptide near the N-Terminus of Duffy.
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 35 kDa band observed in human kidney and human liver lysates (calculated MW of 35.6 kDa according to NP_002027.2). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human kidney, stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. This antibody has also been successfully used on Huamn lung showing endothelial staining. Recommended concentration, 3 μg/mL. FACS: A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.