Western Blotting (WB), Immunohistochemistry (IHC), ELISA
纯化方法
p70 S6 Kinase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
p70 S6 Kinase antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of p70 S6 Kinase.
ELISA: Antibody detection limit dilution >1:128,000. Western Blot: Approximately 65-70 kDa band observed in human muscle and placenta lysates (predicted MW of 60 kDa according to NP_003152.1). Recommended for use at 0.2-1 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows ribosomal staining in DCT cells. Recommended concentration, 1-2 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
RPS6KB1
(Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))