Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
特异性
GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
纯化方法
GAPDH antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
GAPDH antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of GAPDH.
ELISA: Antibody detection limit dilution 1:2000. Western Blot: Approximately 35 kDa band observed in lysates of human tonsil, mouse spleen, and cell lines HeLa, A431 and 293 (calculated MW of 36.1 kDa according to NP_002037). Recommended concentration: 0.01-0.03 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.