The immunizong peptide represents part of the extracellular domain.
纯化方法
NOTCH4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
NOTCH4 antibody was raised against a 14 amino acid synthetic peptide near the internal region of NOTCH4 (aa 1261-1273).
ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 27 kDa band in all human, mouse and rat tissue and cell line lysates tested after 1 μg/mL antibody staining. The 27 kDa band was successfully blocked by incubation with the immunizing peptide.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.