Nestin 抗体
(2 references)Quick Overview for Nestin 抗体 (ABIN2452056)
抗原
See all Nestin (NES) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Specific to mouse Nestin.
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交叉反应 （详细）
- Not tested in other species.
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产品特性
- The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.
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纯化方法
- Purified
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过滤
- Sterile filtered
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免疫原
- Mouse E16 embryonic cerebral cortex extracts
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亚型
- IgG2b kappa
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anti-Nestin (NES) (AA 178-399) antibody
NES 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal D10 unconjugated
anti-Nestin (NES) antibodyNES 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 小鼠 Monoclonal 2C2F7 unconjugated
anti-Nestin (NES) (AA 252-290), (N-Term) antibodyNES 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 1389-1416) antibodyNES 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB16253 unconjugated
anti-Nestin (NES) (AA 698-844) antibodyNES 适用: 人 IHC (f) 宿主: 小鼠 Monoclonal NES-2911 unconjugated
anti-Nestin (NES) (AA 1484-1500) antibodyNES 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 1389-1416) antibodyNES 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 681-750) antibodyNES 适用: 人, 小鼠, 大鼠 ELISA, FACS, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 1392-1621) antibodyNES 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 419-588) antibodyNES 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 1A9G12 unconjugated
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应用备注
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1. Immunocytochemistry
2. Immunohistochemistry
This antibody doesn't work in immunoblotting.
Other applications are not tested.
This antibody is very useful for immunostaining of mouse embryonic brain because it is rat antibody. Rat antibody has very low background in immunostaining using mouse tissues and is also useful for double-staining with mouse and rabbit antibodies. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, 50 % glycerol
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储存液
- Azide free
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储存条件
- -20 °C
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: "Nestin in central nervous system cells." in: Neuroscience and behavioral physiology, Vol. 38, Issue 2, pp. 165-9, (2008) (PubMed).
: "Identification of major cell classes in the developing mammalian nervous system." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 5, Issue 12, pp. 3310-28, (1986) (PubMed).
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: "Nestin in central nervous system cells." in: Neuroscience and behavioral physiology, Vol. 38, Issue 2, pp. 165-9, (2008) (PubMed).
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- Nestin (NES)
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别名
- Nestin
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背景
- Nestin is an intermediate filament protein that is expressed in stem cells and progenitor cells in the mammalian central nervous system (CNS) during development. Nestin is replaced in the adult organism by other intermediate filament proteins, however, it may be re-expressed under certain pathological conditions such as ischemia, inflammation, brain injury, and neoplastic transformation. Nestin has been detected in many kinds of tumors, especially in tumors derived from the CNS, therefore it is considered to be a marker for cancer stem cells in neurogenic tumors.
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UniProt
- Q6P5H2
抗原
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