p53 抗体 (C-Term, pSer392)
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Quick Overview for p53 抗体 (C-Term, pSer392) (ABIN238404)
抗原
See all p53 (TP53) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 378-393, C-Term, pSer392
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特异性
- This p53 antibody FP3.2 [FPS392] reacts with Human p53 tumour suppressor protein phosphorylated at CKII site (Ser392).
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交叉反应 （详细）
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Species reactivity (expected):Rabbit (85 %), Canine (85 %), Guinea pig (85 %), Monkey (92 %), Pig (85 %).
Species reactivity (tested):Human, Mouse. -
产品特性
- Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13
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纯化方法
- Protein A Affinity Chromatography
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免疫原
- KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P] corresponding to amino acids378-393 representing the C-terminus of Human p53.
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亚型
- IgG1
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anti-Tumor Protein P53 (TP53) (N-Term) antibody
TP53 适用: 人 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) antibodyKO Validated TP53 适用: 人 WB, IF, IP, IHC (p), ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (pSer392) antibodyTP53 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 301-393) antibodyTP53 适用: 人, 小鼠, 大鼠, 山羊 WB, ELISA, FACS, IHC (p), ICC, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 94-201) antibodyTP53 适用: 人 WB, ELISA, IF, IP, IHC (p) 宿主: 小鼠 Monoclonal 2C3 unconjugated
anti-Tumor Protein P53 (TP53) (AA 94-201) antibodyTP53 适用: 人 WB, ELISA, IHC (p), PLA, RNAi 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Tumor Protein P53 (TP53) (pSer20) antibodyTP53 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (acLys319) antibodyTP53 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (pSer15) antibodyTP53 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) antibodyTP53 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blotting: 1 μg/mL. Immunohistochemistry on Paraffin Sections): 10 μg/mLStaining technique: Standard ABC technique (DAB+)Pretreatment: High temperature antigen retrieval (microwave, pressure cooker) in 10 mMcitrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0. Incubation Time: 1 hour at RT or overnight at 4 °CPositive Tissue: breast carcinoma with high level of wild-type p53.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH ~7.4, 15 mM Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- p53 (TP53) (Tumor Protein P53 (TP53))
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别名
- p53 (TP53)
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背景
- The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
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基因ID
- 7157
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UniProt
- P04637
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途径
- p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
抗原
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