The antibody 5A6G7 allows to discriminate between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.
交叉反应 (详细)
Species reactivity (tested):Human
产品特性
Synonyms: HLA-6.0, HLAG, MHC class I antigen G, HLA class I histocompatibility antigen
纯化方法
Protein A chromatography
免疫原
C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupledto ovalbumin.
Western Blotting (Reducing Conditions): 12 % AA SDS-PAGE. Positive Control: JEG-3 cell lysate. Flow Cytometry. Immunohistochemistry on Paraffin and Frozen Sections: 10-20 μg/mL. Positive tissue: placenta tissue. Immunocytochemistry. ELISA: The antibody 5A6G7 has been tested as the capture antibody in a sandwich ELISAfor analysis of soluble HLA-G in combination with antibody ABIN238525. Positive Control: HeLa/HLA-G5 transfectants cell lysate. HeLa/HLA-G5 cell supernatant. Negative Control: HeLa cell lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.4 with 15 mM Sodium Azide as preservative
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
有效期
12 months
抗原
HLAG
(HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
MHC-G antibody, B-F antibody, B-F-S04 antibody, B-F-S05 antibody, B-F-S06 antibody, B-F-S07 antibody, B-FI antibody, B-FIV antibody, BF2 antibody, BFa2 antibody, BFw-03 antibody, BFw-05 antibody, BFz-01 antibody, major histocompatibility complex, class I, G antibody, MHC BF1 class I antibody, HLA-G antibody, BF1 antibody
背景
HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.Synonyms: HLA class I histocompatibility antigen, HLA-6.0, HLAG, MHC class I antigen G