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c-MET 抗体 (pTyr1349, pTyr1356)

Name: c-MET 抗体 (pTyr1349, pTyr1356)
SKU: ABIN233821
Availability: OutOfStock

MET 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

Western blot using ’s affinity purified anti-c-Met pY1349pY1356 antibody shows detection of phosphorylated c-Met. (c-MET 抗体 (pTyr1349, pTyr1356))

Western blot using affinity purified anti-c-Met pY1349pY1356 antibody shows detection of phosphorylated c-Met. (c-MET 抗体 (pTyr1349, pTyr1356))

2 images

产品编号 ABIN233821

Supplier Product No.: 600-401-989

发货至: 中国

Datasheet as PDF

抗原 See all c-MET (MET) 抗体

c-MET (MET) (Met Proto-Oncogene (MET))
抗原表位
41

28

27

23

12

10

9

7

7

7

5

4

4

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pTyr1349, pTyr1356
适用
222

129

97

5

4

4

4

4

2

2

2

1

1

1

1
人
宿主
192

38

3

2

2
兔
Compatible Secondaries
克隆类型
187

50
多克隆
标记
125

18

10

10

7

7

7

7

7

7

5

5

2

2

2

2

2

2

2

2

2

2

2
This c-MET antibody is un-conjugated
应用范围
153

95

39

29

28

20

15

14

13

8

8

5

4

4

1

1

1

1

1

1
Western Blotting (WB), ELISA

Supplier Product No.

600-401-989

Supplier

Rockland

原理

c-Met phospho Y1349 / pY1356 Antibody

交叉反应（详细）

Antibody is specific for human c-Met protein phosphorylated at Y1349 and Y1356.

产品特性

Synonyms: rabbit anti-c-Met pY1349/pY1356 Antibody, rabbit anti-c-Met dual phosphorylated Antibody, HGF receptor antibody, HGF/SF receptor antibody, HGFR antibody, MET antibody, Met proto oncogene tyrosine kinase antibody, SF receptor, Tyrosine-protein kinase Met, Scatter factor receptor, Proto-oncogene c-Met, Hepatocyte growth factor receptor

纯化方法

Phospho c-MET Antibody was produced from monospecific antiserum by immunoaffinity chromatography using dual-phospho-peptide coupled to agarose beads followed by cross-adsorption against nonphospho-peptide.

过滤

Sterile filtered

免疫原

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y1349 and Y1356 of human c-Met protein.
Immunogen Type: Conjugated Peptide

亚型

IgG

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应用备注

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 150 kDa in size corresponding to phosphorylated c-Met protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human c-Met pY1349pY1356 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 1:93,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.01 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store Phospho antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
抗原

c-MET (MET) (Met Proto-Oncogene (MET))

别名

MET (MET 产品)

背景

Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Anti-c-MET is the receptor for hepatocyte growth factor (also known as scatter factor, HGF/SF), and belongs to the tyrosine kinase superfamily. Interaction of c-Met with HGF results in autophosphorylation of c-Met at multiple tyrosines. Phosphorylation of Y1234/1235 in the c-Met kinase domain is critical to kinase activation. When phosphorylated, Y1349 and Y1356, along with surrounding amino acids, form a unique bidentate docking site for substrates such as Gab1, Grb2, phosphatidylinositol 3-kinase (PI3K) and others. C-Met mainly uses the Gab1 scaffolding adaptor in its initial step of signal transmission. Well-characterized downstream signaling pathways that are activated by c-Met include the ERK/MAPK, PI3K-Akt/PKB, Crk-Rap and Rac-Pak pathways, resulting in proliferation and increased cell survival. Anti-Phospho cMET was developed with NCI and is ideal for Cancer Research.

基因ID

4233, 42741655

UniProt

P08581

途径

RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor