c-MET 抗体 (pTyr1234, Tyr1235)
Quick Overview for c-MET 抗体 (pTyr1234, Tyr1235) (ABIN228894)
抗原
See all c-MET (MET) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr1234, Tyr1235
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特异性
- Detects Met only when phosphorylated at tyrosine 1234/1235 (Mr 145kD). This antibody cross-reacts slightly with activated PDGF, insulin and FGF receptors, but it does not cross-react with other tyrosine-phosphorylated proteins. Species cross-reactivity: Human, mouse and monkey. Species sequence homology: rat 100 % .
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr1234/1235 of human Met.
Type of Immunogen: Synthetic phospho-peptide -
亚型
- IgG
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应用备注
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Approved: ELISA, ICC (1:50), IP (1:50), WB (1:1000)
Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- c-MET (MET) (Met Proto-Oncogene (MET))
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别名
- c-Met
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背景
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Name/Gene ID: MET
Subfamily: HGF Receptor/MET
Family: Protein Kinase
Synonyms: MET, AUTS9, C-Met, HGF/SF receptor, HGFR, Met kinase, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, HGF receptor, RCCP2, Tyrosine-protein kinase Met -
基因ID
- 4233
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UniProt
- P08581
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途径
- RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
抗原
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