c-MET 抗体 (pTyr1234, Tyr1235)
Quick Overview for c-MET 抗体 (pTyr1234, Tyr1235) (ABIN224750)
抗原
See all c-MET (MET) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr1234, Tyr1235
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特异性
- Detects endogenous human HGF R/c-MET phosphorylated at Y1234/Y1235.
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纯化方法
- Immunoaffinity purified
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免疫原
- Phosphopeptide containing human HGF R/c-MET Y1234/1235 sites.
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亚型
- IgG
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应用备注
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Approved: Flo, WB
Usage: Western Blot: An antibody concentration of 0.5 μg/mL is recommended. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 L. Following a 30 minute incubation, cells should be washed in an isotonic phosphate buffer (supplemented with 0.5 % BSA) prior to the addition of a secondary developing reagent. The binding of unlabeled polyclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL solution of a secondary developing reagent such as anti-rabbit IgG conjugated to a fluorochrome. Cells should be washed for a final time prior to flow cytometric analysis. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- Lyophilized.
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- c-MET (MET) (Met Proto-Oncogene (MET))
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别名
- c-Met
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背景
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Name/Gene ID: MET
Subfamily: HGF Receptor/MET
Family: Protein Kinase
Synonyms: MET, AUTS9, C-Met, HGF/SF receptor, HGFR, Met kinase, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, HGF receptor, RCCP2, Tyrosine-protein kinase Met -
基因ID
- 4233
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UniProt
- P08581
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途径
- RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
抗原
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