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c-MET 抗体 (pTyr1234, Tyr1235)

The 兔 多克隆 anti-c-MET antibody is suitable to detect c-MET in samples from 人. It has been validated for WB 和 FACS.
产品编号 ABIN224750
发货至: 中国
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北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Quick Overview for c-MET 抗体 (pTyr1234, Tyr1235) (ABIN224750)

抗原

See all c-MET (MET) 抗体
c-MET (MET) (Met Proto-Oncogene (MET))

适用

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克隆类型

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多克隆

标记

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This c-MET antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS)
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    pTyr1234, Tyr1235

    特异性

    Detects endogenous human HGF R/c-MET phosphorylated at Y1234/Y1235.

    纯化方法

    Immunoaffinity purified

    免疫原

    Phosphopeptide containing human HGF R/c-MET Y1234/1235 sites.

    亚型

    IgG
  • 应用备注

    Approved: Flo, WB

    Usage: Western Blot: An antibody concentration of 0.5 μg/mL is recommended. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 L. Following a 30 minute incubation, cells should be washed in an isotonic phosphate buffer (supplemented with 0.5 % BSA) prior to the addition of a secondary developing reagent. The binding of unlabeled polyclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL solution of a secondary developing reagent such as anti-rabbit IgG conjugated to a fluorochrome. Cells should be washed for a final time prior to flow cytometric analysis.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    deionized water

    浓度

    Lot specific

    缓冲液

    Lyophilized.
  • 抗原

    c-MET (MET) (Met Proto-Oncogene (MET))

    别名

    c-Met

    背景

    Name/Gene ID: MET
    Subfamily: HGF Receptor/MET
    Family: Protein Kinase

    Synonyms: MET, AUTS9, C-Met, HGF/SF receptor, HGFR, Met kinase, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, HGF receptor, RCCP2, Tyrosine-protein kinase Met

    基因ID

    4233

    UniProt

    P08581

    途径

    RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
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