(4 references)-
- 抗原 See all Nitrotyrosine 抗体
- Nitrotyrosine
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Nitrotyrosine antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA)
- 过滤
- 0.2 μm filtered
- 克隆位点
- HM-11
- Top Product
- Discover our top product Nitrotyrosine Primary Antibody
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anti-Nitrotyrosine antibody
WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 unconjugated
anti-Nitrotyrosine antibody (HRP)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 HRP
anti-Nitrotyrosine antibody (FITC)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 FITC
anti-Nitrotyrosine antibody (Biotin)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Biotin
anti-Nitrotyrosine antibody (PE)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 PE
anti-Nitrotyrosine antibody (APC)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 APC
anti-Nitrotyrosine antibody (PerCP)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 PerCP
anti-Nitrotyrosine antibody (Atto 488)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Atto 488
anti-Nitrotyrosine antibody (Atto 594)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Atto 594
anti-Nitrotyrosine antibody (Atto 390)WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Atto 390
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- 应用备注
- For immuno histology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Warning: keep sterile, do not add sodium azide or any nitric containing preservative since this will affect the antibody!
- 限制
- 仅限研究用
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- 缓冲液
- PBS, containing 0.1 % bovine serum albumin.
- 储存条件
- 4 °C
- 储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
- 有效期
- 12 months
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: "Nitrotyrosine in plasma of celiac disease patients as detected by a new sandwich ELISA." in: Free radical biology & medicine, Vol. 25, Issue 8, pp. 953-63, (1999) (PubMed).
: "Evidence for in vivo peroxynitrite production in human acute lung injury." in: American journal of respiratory and critical care medicine, Vol. 151, Issue 4, pp. 1250-4, (1995) (PubMed).
: "Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry." in: Biological chemistry Hoppe-Seyler, Vol. 375, Issue 2, pp. 81-8, (1994) (PubMed).
: "Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 4, pp. 1620-4, (1990) (PubMed).
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: "Nitrotyrosine in plasma of celiac disease patients as detected by a new sandwich ELISA." in: Free radical biology & medicine, Vol. 25, Issue 8, pp. 953-63, (1999) (PubMed).
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- 抗原
- Nitrotyrosine
- Abstract
- Nitrotyrosine 产品
- 物质类
- Chemical
- 背景
- This highly specific monoclonal antibody HM.11 reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques. Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production.
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