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Nitrotyrosine 抗体 (APC)

WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 APC
产品编号 ABIN2486191
发货至: 中国
  • 抗原 See all Nitrotyrosine 抗体
    Nitrotyrosine
    适用
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    宿主
    • 26
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    小鼠
    克隆类型
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    单克隆
    标记
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    This Nitrotyrosine antibody is conjugated to APC
    应用范围
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    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Antibody Array (AA)
    特异性
    Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.
    纯化方法
    Protein G Purified
    免疫原
    3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin
    克隆位点
    39B6
    亚型
    IgG2a
    Top Product
    Discover our top product Nitrotyrosine Primary Antibody
  • 应用备注
    • WB (1:1400)
    • IHC (1:100)
    • optimal dilutions for assays should be determined by the user.
    说明

    0.7 μg/ml of ABIN2486191 was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Conjugated antibodies should be stored at 4°C
  • 抗原
    Nitrotyrosine
    Abstract
    Nitrotyrosine 产品
    物质类
    Chemical
    背景
    Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).
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