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Nitrotyrosine 抗体

Name: Nitrotyrosine 抗体
SKU: ABIN1385080
Availability: OutOfStock

适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse intestine tissue labeled with Anti-Nitrotyrosine Polyclonal Antibody, Unconjugated (ABIN1385080) at 1:200, followed by conjugation to the secondary antibody and DAB staining

IHC

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Nitrotyrosine Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

Hela probed with Nitrotyrosine Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Cpnjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

FACS

3 images

(5 references)

产品编号 ABIN1385080

发货至: 中国

Datasheet as PDF

抗原 See all Nitrotyrosine 抗体

Nitrotyrosine
适用
19

15

15

2
人, 小鼠, 大鼠
宿主
27

22

5
兔
Compatible Secondaries
克隆类型
27

27
多克隆
标记
21

7

4

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Nitrotyrosine antibody is un-conjugated
应用范围
52

27

25

20

19

15

12

12

11

11

9

8

4

3

2

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

交叉反应

人, 小鼠, 大鼠

交叉反应（详细）

Nitrotyrosine

纯化方法

Purified by Protein A.

免疫原

KLH conjugated to Nitrotyrosine

亚型

IgG

Top Product

Discover our top product Nitrotyrosine Primary Antibody
anti-Nitrotyrosine antibody
WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 unconjugated

anti-Nitrotyrosine antibody (Biotin)
WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Biotin

anti-Nitrotyrosine antibody (HRP)
WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 HRP

anti-Nitrotyrosine antibody (FITC)
WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 FITC

anti-Nitrotyrosine antibody (Alkaline Phosphatase (AP))
WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Alkaline Phosphatase (AP)

anti-Nitrotyrosine antibody (PE)
WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 PE

anti-Nitrotyrosine antibody (PerCP)
WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 PerCP

anti-Nitrotyrosine antibody (APC)
WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 APC

anti-Nitrotyrosine antibody (Atto 594)
WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Atto 594

anti-Nitrotyrosine antibody (Atto 488)
WB, ELISA, IP, IHC, FACS, ICC, IF, AA 宿主: 小鼠 Monoclonal 39B6 Atto 488

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Li, Du, Yuan, Han, Dong, Chen, Wu, Zhang, Xu, Han, Zhang, Xia: "Hepatic hypoxia-inducible factors inhibit PPARα expression to exacerbate acetaminophen induced oxidative stress and hepatotoxicity." in: Free radical biology & medicine, Vol. 110, pp. 102-116, (2017) (PubMed).

Zhang, Song, Pang, Zhang, Wan, Yuan, Wu, Liu: "Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice." in: World journal of gastroenterology : WJG, Vol. 21, Issue 14, pp. 4195-209, (2015) (PubMed).

Chen, Kan: "Down-regulation of superoxide dismutase 1 by PMA is involved in cell fate determination and mediated via protein kinase D2 in myeloid leukemia cells." in: Biochimica et biophysica acta, Vol. 1853, Issue 10 Pt A, pp. 2662-75, (2015) (PubMed).

Wang, Su, Wang, Zhang, Zhang, Niu, Guo, Li, Jiang, Liu, Guo: "Tert-butylhydroquinone ameliorates doxorubicin-induced cardiotoxicity by activating Nrf2 and inducing the expression of its target genes." in: American journal of translational research, Vol. 7, Issue 10, pp. 1724-35, (2015) (PubMed).

Vendrov, Vendrov, Smith, Yuan, Sumida, Robidoux, Runge, Madamanchi: "NOX4 NADPH Oxidase-Dependent Mitochondrial Oxidative Stress in Aging-Associated Cardiovascular Disease." in: Antioxidants & redox signaling, (2015) (PubMed).
抗原

Nitrotyrosine

Abstract

Nitrotyrosine 产品

物质类

Chemical

背景

Synonyms: NULL
Background: Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques and chronic renal failure.