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- 抗原 See all MME 抗体
- MME (Membrane Metallo-Endopeptidase (MME))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MME antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- 4ACB-CALLA reacts with CD10, 100 kDa common acute lymphoblastic leukemia antigen (CALLA), which is expressed on lymphoid precursors, germinal center B cells, and peripheral blood granulocytes. CD10 is a regulator of B cell growth and proliferation. CD10 antibody is used in conjunction with other reagents in the phenotyping of leukemia.*CD10 is a phenotyping marker of leukemic cells that may have prognostic significance in leukemia clinical application.
- 克隆位点
- 4ACB-CALLA
- 亚型
- IgG1
- Top Product
- Discover our top product MME Primary Antibody
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anti-Membrane Metallo-Endopeptidase (MME) (AA 52-352) antibody
MME 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Membrane Metallo-Endopeptidase (MME) (AA 52-246) antibodyMME 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 7D4B1 unconjugated
anti-Membrane Metallo-Endopeptidase (MME) (AA 501-600) antibodyMME 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Membrane Metallo-Endopeptidase (MME) (AA 382-538) antibodyMME 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Membrane Metallo-Endopeptidase (MME) (AA 297-483) antibodyMME 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal MME-1870 unconjugated
anti-Membrane Metallo-Endopeptidase (MME) (AA 286-428) antibodyMME 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Membrane Metallo-Endopeptidase (MME) (AA 297-483) antibodyMME 适用: 人 WB, IHC, ELISA, StM, Coat 宿主: 小鼠 Monoclonal MME-1892 unconjugated
anti-Membrane Metallo-Endopeptidase (MME) antibody (FITC)MME 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-78 FITC
anti-Membrane Metallo-Endopeptidase (MME) (AA 549-750) antibodyMME 适用: 人 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 6G12E12 unconjugated
anti-Membrane Metallo-Endopeptidase (MME) (AA 52-750) antibodyMME 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- MME (Membrane Metallo-Endopeptidase (MME))
- 别名
- CD10 (MME 产品)
- 别名
- CALLA antibody, CD10 antibody, NEP antibody, SFE antibody, 6030454K05Rik antibody, C85356 antibody, Nep antibody, neprilysin antibody, membrane metalloendopeptidase antibody, membrane metallo endopeptidase antibody, membrane metallo-endopeptidase antibody, MME antibody, Mme antibody
- 物质类
- Chemical
- 途径
- RTK signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration
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