FCGR1A 抗体 (FITC)
-
- 抗原 See all FCGR1A 抗体
- FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This FCGR1A antibody is conjugated to FITC
-
应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 产品特性
- 4A10.1 reacts with high affinity receptor for IgG (FcyRI), a 75 kDa type 1 trasmembrane glycoprotein. CD64 is expressed on monocytes and macrophages but not on lymphocytes or resting granulocytes. CD64 play a role in phagocytosis, and antibody dependent cellular cytotoxicity ( ADCC). It also participates in cytokine and superoxide release.
- 克隆位点
- 4A10-1
- 亚型
- IgG1
- Top Product
- Discover our top product FCGR1A Primary Antibody
-
anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 200-292) antibody
FCGR1A 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 16-145) antibodyFCGR1A 适用: 人 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 5F5F12 unconjugated
anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) antibodyFCGR1A 适用: 人, Non-Human Primate FACS, WB, ICC, IP, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal 10-1 unconjugated
anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) antibodyFCGR1A 适用: 人, Non-Human Primate FACS, WB, ICC, IP, IHC (p), Func, IHC (fro) 宿主: 小鼠 Monoclonal 10-1 unconjugated
anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) antibody (FITC)FCGR1A 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 10-1 FITC
anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) antibody (APC)FCGR1A 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 10-1 APC
anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 230-280) antibodyFCGR1A 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 35-235) antibodyFCGR1A 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 16-115) antibodyFCGR1A 适用: 人 ELISA, PLA 宿主: 小鼠 Monoclonal 1D3 unconjugated
anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (Internal Region) antibodyFCGR1A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
-
- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
-
- 抗原
- FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))
- 别名
- CD64 (FCGR1A 产品)
- 别名
- CD64 antibody, CD64A antibody, FCRI antibody, IGFR1 antibody, FcgammaRI antibody, Fcgr1 antibody, Fcgr1b antibody, AI323638 antibody, AV092959 antibody, IGGHAFC antibody, FCGR1A antibody, Fc fragment of IgG receptor Ia antibody, Fc receptor, IgG, high affinity I antibody, FCGR1A antibody, Fcgr1a antibody, Fcgr1 antibody
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
-
