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FCGR1A 抗体 (FITC)

Name: FCGR1A 抗体 (FITC)
Brand: antibodies-online
SKU: ABIN1302937
Availability: OutOfStock

FCGR1A 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 10-1 FITC

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD64 (10. (FCGR1A 抗体 (FITC))

FACS

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD64 (10. (FCGR1A 抗体 (FITC))

FACS

2 images

(10 references)

产品编号 ABIN1302937

发货至: 中国

Datasheet as PDF

抗原 See all FCGR1A 抗体

FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))
适用
105

25

19

13

5

5

4

4

4

1
人, Non-Human Primate
宿主
70

40

1
小鼠
Compatible Secondaries
克隆类型
69

40

2
单克隆
标记
40

14

12

8

7

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This FCGR1A antibody is conjugated to FITC
应用范围
77

65

31

25

20

19

12

11

9

8

6

3

3

2

1

1

1
Flow Cytometry (FACS)

特异性

The mouse monoclonal antibody 10.1 recognizes an extracellular epitope on CD64/FcgammaRI, a 72 kDa single chain type I glycoprotein, that is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The epitope is sensitive to formalin fixation.

交叉反应（详细）

Human, Non-Human Primates

纯化方法

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Rheumatoid synovial fluid cells and fibronectin purified human monocytes

克隆位点

10-1

亚型

IgG1 kappa

Top Product

Discover our top product FCGR1A Primary Antibody
anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 200-292) antibody
FCGR1A 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 16-145) antibody
FCGR1A 适用: 人 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 5F5F12 unconjugated

anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) antibody
FCGR1A 适用: 人, Non-Human Primate FACS, WB, ICC, IP, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal 10-1 unconjugated

anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) antibody
FCGR1A 适用: 人, Non-Human Primate FACS, WB, ICC, IP, IHC (p), Func, IHC (fro) 宿主: 小鼠 Monoclonal 10-1 unconjugated

anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) antibody (APC)
FCGR1A 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 10-1 APC

anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 230-280) antibody
FCGR1A 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 35-235) antibody
FCGR1A 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (AA 16-115) antibody
FCGR1A 适用: 人 ELISA, PLA 宿主: 小鼠 Monoclonal 1D3 unconjugated

anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) (Internal Region) antibody
FCGR1A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A) antibody
FCGR1A 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 3D3 unconjugated

应用备注

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

说明

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

限制

仅限研究用
溶解方式

No reconstitution is necessary.

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Devaraj, Davis, Simon, Jialal: "CRP promotes monocyte-endothelial cell adhesion via Fcgamma receptors in human aortic endothelial cells under static and shear flow conditions." in: American journal of physiology. Heart and circulatory physiology, Vol. 291, Issue 3, pp. H1170-6, (2006) (PubMed).

Devaraj, Du Clos, Jialal: "Binding and internalization of C-reactive protein by Fcgamma receptors on human aortic endothelial cells mediates biological effects." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 25, Issue 7, pp. 1359-63, (2005) (PubMed).

Roura-Mir, Wang, Cheng, Matsunaga, Dascher, Peng, Fenton, Kirschning, Moody: "Mycobacterium tuberculosis regulates CD1 antigen presentation pathways through TLR-2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 3, pp. 1758-66, (2005) (PubMed).

Beekman, Bakema, van der Linden, Tops, Hinten, van Vugt, van de Winkel, Leusen: "Modulation of FcgammaRI (CD64) ligand binding by blocking peptides of periplakin." in: The Journal of biological chemistry, Vol. 279, Issue 32, pp. 33875-81, (2004) (PubMed).

Sánchez-Torres, García-Romo, Cornejo-Cortés, Rivas-Carvalho, Sánchez-Schmitz: "CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells." in: International immunology, Vol. 13, Issue 12, pp. 1571-81, (2001) (PubMed).

Brichard, Varis, Latinne, Deneys, de Bruyere, Leveugle, Cornu: "Intracellular cytokine profile of cord and adult blood monocytes." in: Bone marrow transplantation, Vol. 27, Issue 10, pp. 1081-6, (2001) (PubMed).

Fadlon, Vordermeier, Pearson, Mire-Sluis, Dumonde, Phillips, Fishlock, Brown: "Blood polymorphonuclear leukocytes from the majority of sickle cell patients in the crisis phase of the disease show enhanced adhesion to vascular endothelium and increased expression of CD64." in: Blood, Vol. 91, Issue 1, pp. 266-74, (1998) (PubMed).

Hashimoto, Yamada, Motoyoshi, Akagawa: "Enhancement of macrophage colony-stimulating factor-induced growth and differentiation of human monocytes by interleukin-10." in: Blood, Vol. 89, Issue 1, pp. 315-21, (1997) (PubMed).

Jayaram, Buckle, Hogg: "The Fc receptor, FcRI, and other activation molecules on human mononuclear phagocytes after treatment with interferon-gamma." in: Clinical and experimental immunology, Vol. 75, Issue 3, pp. 414-20, (1989) (PubMed).

Dougherty, Selvendran, Murdoch, Palmer, Hogg: "The human mononuclear phagocyte high-affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1." in: European journal of immunology, Vol. 17, Issue 10, pp. 1453-9, (1987) (PubMed).
抗原

FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

别名

CD64 (FCGR1A 产品)

别名

CD64 antibody, CD64A antibody, FCRI antibody, IGFR1 antibody, FcgammaRI antibody, Fcgr1 antibody, Fcgr1b antibody, AI323638 antibody, AV092959 antibody, IGGHAFC antibody, FCGR1A antibody, Fc fragment of IgG receptor Ia antibody, Fc receptor, IgG, high affinity I antibody, FCGR1A antibody, Fcgr1a antibody, Fcgr1 antibody

背景

Fc fragment of IgG receptor Ia,CD64 (FcgammaRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding alpha subunit and promiscuous gamma subunit, which is indispensable for tyrosine-based signaling. However, even the alpha subunit can transduce signals leading to cellular effector functions. The isoform FcgammaRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcgammaRIb and FcgammaRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo.,FcRI, IGFR1, FcgR1A

基因ID

2209

UniProt

P12314

途径

Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process