CHEK2 抗体
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- 抗原 See all CHEK2 抗体
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
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适用
- 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CHEK2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 特异性
- Recognizes human CHK2, Mr 67kD. Species cross-reactivity: mouse. Species sequence homology: rat.
- 纯化方法
- Protein G purified
- 免疫原
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Mixture of two GST fusion proteins corresponding to amino acids NT, 1-223 and CT 217-543 of human CHK2.
Type of Immunogen: Fusion protein - 亚型
- IgG1
- Top Product
- Discover our top product CHEK2 Primary Antibody
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- 应用备注
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Approved: ICC, IP, WB (0.1 - 1 μg/mL)
Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Immunoprecipitation: Detects HK2 from HeLa cells. Reported by an independent lab. Immunocytochemistry: Detects CHK2 in HeLa cells. Reported by an independent lab. Western Blot: 0.1-1.0 μg/mL detects CHK2 in lysates from Jurkat, HeLa, 3T3/A31, and A431 cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CHK2 (0.5 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive control: J9000: Jurkat cell lysate. Add 2.5 μL of 2-mercaptoethanol/ 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- 抗原
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
- 别名
- CHEK2 / CHK2 (CHEK2 产品)
- 别名
- CDS1 antibody, CHK2 antibody, HuCds1 antibody, LFS2 antibody, PP1425 antibody, RAD53 antibody, hCds1 antibody, fa66f08 antibody, wu:fa66f08 antibody, zgc:55865 antibody, Cds1 antibody, HUCDS1 antibody, Rad53 antibody, Chk2 antibody, cds1 antibody, chek2 antibody, chk2 antibody, hucds1 antibody, lfs2 antibody, pp1425 antibody, rad53 antibody, checkpoint kinase 2 antibody, serine/threonine-protein kinase chk2 antibody, checkpoint kinase 2 L homeolog antibody, CHEK2 antibody, chek2 antibody, Chek2 antibody, MCYG_07308 antibody, chek2.L antibody
- 背景
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Name/Gene ID: CHEK2
Subfamily: CDS1
Family: Protein Kinase
Synonyms: CHEK2, BA444G7, Cds1 homolog, Checkpoint kinase 2, Checkpoint-like protein CHK2, CHK2 checkpoint homolog, CHK2, HCds1, LFS2, RAD53, Rad53 homolog, HCHK2, HuCds1, PP1425 - 基因ID
- 11200
- UniProt
- O96017
- 途径
- p53 Pathway, Apoptosis, Cell Division Cycle
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