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CHEK2 抗体

CHEK2 适用: 人, 小鼠, 大鼠 WB, IP, ICC 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN210859
发货至: 中国
  • 抗原 See all CHEK2 抗体
    CHEK2 (Checkpoint Kinase 2 (CHEK2))
    适用
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    人, 小鼠, 大鼠
    宿主
    • 232
    • 19
    • 1
    • 1
    小鼠
    克隆类型
    • 226
    • 27
    单克隆
    标记
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    This CHEK2 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    特异性
    Recognizes human CHK2, Mr 67kD. Species cross-reactivity: mouse. Species sequence homology: rat.
    纯化方法
    Protein G purified
    免疫原
    Mixture of two GST fusion proteins corresponding to amino acids NT, 1-223 and CT 217-543 of human CHK2.

    Type of Immunogen: Fusion protein
    亚型
    IgG1
    Top Product
    Discover our top product CHEK2 Primary Antibody
  • 应用备注
    Approved: ICC, IP, WB (0.1 - 1 μg/mL)

    Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Immunoprecipitation: Detects HK2 from HeLa cells. Reported by an independent lab. Immunocytochemistry: Detects CHK2 in HeLa cells. Reported by an independent lab. Western Blot: 0.1-1.0 μg/mL detects CHK2 in lysates from Jurkat, HeLa, 3T3/A31, and A431 cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CHK2 (0.5 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive control: J9000: Jurkat cell lysate. Add 2.5 μL of 2-mercaptoethanol/ 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • 抗原
    CHEK2 (Checkpoint Kinase 2 (CHEK2))
    别名
    CHEK2 / CHK2 (CHEK2 产品)
    别名
    CDS1 antibody, CHK2 antibody, HuCds1 antibody, LFS2 antibody, PP1425 antibody, RAD53 antibody, hCds1 antibody, fa66f08 antibody, wu:fa66f08 antibody, zgc:55865 antibody, Cds1 antibody, HUCDS1 antibody, Rad53 antibody, Chk2 antibody, cds1 antibody, chek2 antibody, chk2 antibody, hucds1 antibody, lfs2 antibody, pp1425 antibody, rad53 antibody, checkpoint kinase 2 antibody, serine/threonine-protein kinase chk2 antibody, checkpoint kinase 2 L homeolog antibody, CHEK2 antibody, chek2 antibody, Chek2 antibody, MCYG_07308 antibody, chek2.L antibody
    背景
    Name/Gene ID: CHEK2
    Subfamily: CDS1
    Family: Protein Kinase

    Synonyms: CHEK2, BA444G7, Cds1 homolog, Checkpoint kinase 2, Checkpoint-like protein CHK2, CHK2 checkpoint homolog, CHK2, HCds1, LFS2, RAD53, Rad53 homolog, HCHK2, HuCds1, PP1425
    基因ID
    11200
    UniProt
    O96017
    途径
    p53 Pathway, Apoptosis, Cell Division Cycle
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