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CHEK2 抗体 (AA 481-531)

Name: CHEK2 抗体 (AA 481-531)
Brand: antibodies-online
SKU: ABIN1724714
Availability: OutOfStock

CHEK2 适用: 人 WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 1C12B8 unconjugated

Western blot analysis using CHK2 mouse mAb against cell lysate from various cell types. (CHEK2 抗体 (AA 481-531))

Confocal immunofluorescence analysis of Hela cells using CHK2 mouse mAb (green), showing nuclear localization. (CHEK2 抗体 (AA 481-531))

Immunohistochemical analysis of paraffin-embedded human lung carcinoma (A), liver carcinoma (B), breast carcinoma (C) and kiney carcinoma (D), showing nuclear localization with DAB staining using CHK2 mouse mAb. (CHEK2 抗体 (AA 481-531))

IHC

3 images

(3 references)

产品编号 ABIN1724714

发货至: 中国

Datasheet as PDF

抗原 See all CHEK2 抗体

CHEK2 (Checkpoint Kinase 2 (CHEK2))
抗原表位
30

28

21

17

16

15

15

15

9

8

8

8

8

7

7

6

4

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 481-531
适用
260

96

82

16

14

7

6

5

2

2

2

1

1
人
宿主
242

19

1

1
小鼠
Compatible Secondaries
克隆类型
228

35
单克隆
标记
115

14

12

12

12

12

12

12

10

9

7

7

7

7

7

2

2

2

1

1
This CHEK2 antibody is un-conjugated
应用范围
205

80

79

71

36

36

33

28

28

19

17

5

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

纯化方法

purified

免疫原

Purified recombinant fragment of human CHK2 (aa481-531) expressed in E. coli.

克隆位点

1C12B8

亚型

IgG2b

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anti-Checkpoint Kinase 2 (CHEK2) (AA 1-543) antibody
CHEK2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

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CHEK2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4B7 unconjugated

anti-Checkpoint Kinase 2 (CHEK2) (pSer516) antibody
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anti-Checkpoint Kinase 2 (CHEK2) (pThr68) antibody
CHEK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 2 (CHEK2) (pThr383) antibody
CHEK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 2 (CHEK2) antibody
CHEK2 适用: 人, 猴, 犬 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 10G7 unconjugated

anti-Checkpoint Kinase 2 (CHEK2) antibody
CHEK2 适用: 人, 犬 WB, IHC, IF, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 5C4 unconjugated

anti-Checkpoint Kinase 2 (CHEK2) antibody
CHEK2 适用: 人, 犬 WB, IHC, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 2H5 unconjugated

anti-Checkpoint Kinase 2 (CHEK2) (AA 1-220) antibody
CHEK2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000

限制

仅限研究用
状态

Liquid

缓冲液

Ascitic fluid containing 0.03 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

4°C, -20°C for long term storage
Cybulski, Masojc, Oszutowska, Jaworowska, Grodzki, Waloszczyk, Serwatowski, Pankowski, Huzarski, Byrski, Górski, Jakubowska, Debniak, Wokolorczyk, Gronwald, Tarnowska, Serrano-Fernández, Lubinski et al.: "Constitutional CHEK2 mutations are associated with a decreased risk of lung and laryngeal cancers. ..." in: Carcinogenesis, Vol. 29, Issue 4, pp. 762-5, (2008) (PubMed).

Antoni, Sodha, Collins, Garrett: "CHK2 kinase: cancer susceptibility and cancer therapy - two sides of the same coin?" in: Nature reviews. Cancer, Vol. 7, Issue 12, pp. 925-36, (2007) (PubMed).

Bell, Kim, Godwin, Schiripo, Harris, Haserlat, Wahrer, Haiman, Daly, Niendorf, Smith, Sgroi, Garber, Olopade, Le Marchand, Henderson, Altshuler, Haber, Freedman: "Genetic and functional analysis of CHEK2 (CHK2) variants in multiethnic cohorts." in: International journal of cancer. Journal international du cancer, Vol. 121, Issue 12, pp. 2661-7, (2007) (PubMed).
抗原

CHEK2 (Checkpoint Kinase 2 (CHEK2))

别名

CHK2 (CHEK2 产品)

别名

CDS1 antibody, CHK2 antibody, HuCds1 antibody, LFS2 antibody, PP1425 antibody, RAD53 antibody, hCds1 antibody, fa66f08 antibody, wu:fa66f08 antibody, zgc:55865 antibody, Cds1 antibody, HUCDS1 antibody, Rad53 antibody, Chk2 antibody, cds1 antibody, chek2 antibody, chk2 antibody, hucds1 antibody, lfs2 antibody, pp1425 antibody, rad53 antibody, checkpoint kinase 2 antibody, serine/threonine-protein kinase chk2 antibody, checkpoint kinase 2 L homeolog antibody, CHEK2 antibody, chek2 antibody, Chek2 antibody, MCYG_07308 antibody, chek2.L antibody

背景

Description: CHK2: CHK2 checkpoint homolog (S. pombe). In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Aliases: CDS1, LFS2, CHEK2

分子量

61 kDa

基因ID

11200

HGNC

11200

途径

p53 Pathway, Apoptosis, Cell Division Cycle