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MMP14 抗体 (AA 319-333)

MMP14 适用: 人, 小鼠, 大鼠, Cow, 猴, 山羊, Hamster, 绵羊 WB, ELISA, IHC, IP, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN209030
发货至: 中国
  • 抗原 See all MMP14 抗体
    MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))
    抗原表位
    • 14
    • 13
    • 9
    • 8
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 319-333
    适用
    • 95
    • 33
    • 32
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    人, 小鼠, 大鼠, Cow, 猴, 山羊, Hamster, 绵羊
    宿主
    • 85
    • 10
    • 1
    小鼠
    克隆类型
    • 75
    • 21
    单克隆
    标记
    • 52
    • 8
    • 6
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This MMP14 antibody is un-conjugated
    应用范围
    • 65
    • 46
    • 35
    • 18
    • 13
    • 10
    • 9
    • 6
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    特异性
    Recognizes human MT1-MMP. Very slight cross-reactivity to hMMP-3. Species cross-reactivity: Mouse, rat, rabbit porcine and equine. For unknown reasons this antibody does not react on westerns to MMP-14 [MT1-MMP], Human, Prodomain, Catalytic Domain.
    预测反应
    Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%) Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%) Zebrafish, Xenopus, Medaka (87%) Lizard (80%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Peptide (aa 319-333, CDGNFDTVAMLRGEM) in the hemopexin-like domain of human membrane Type1 matrix metalloproteinase human MT1-MMP also referred to as MMP-14. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Goat, Hamster, Porcine, Bovine, Opossum, Platypus (100%), Elephant, Panda, Stickleback, Turkey, Bat, Dog, Horse, Rabbit, Chicken, Pufferfish (93%), Zebrafish, Xenopus, Medaka (87%), Lizard (80%).
    亚型
    IgG3 kappa
    Top Product
    Discover our top product MMP14 Primary Antibody
  • 应用备注
    Approved: ELISA, IHC, IHC-Fr, IHC-P, IP, WB (10 μg/mL)

    Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry, and Immunoprecipitation. Western Blot: 10 μg/mL, membrane preparations, MD-MB-231 cells pretreated with 1-5 μg/mL ConA for 24-48 hours for positive control. 60-65kD proteins are identified. When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59kD. Immunohistochemistry: Frozen sections, traditional formalin fixation problematic. Paraffin-embedded tissue sections with PLP fixation required.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS,  pH 7.0, 2 % BSA (protease free).
    储存条件
    4 °C,-20 °C
    储存方法
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原
    MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))
    别名
    MMP14 / MMP-14 (MMP14 产品)
    别名
    MMP1 antibody, MMP-14 antibody, MMP-X1 antibody, MT-MMP antibody, MT-MMP 1 antibody, MT1-MMP antibody, MT1MMP antibody, MTMMP1 antibody, mmp-x1 antibody, mt1-mmp antibody, mtmmp1 antibody, AI325305 antibody, MT-MMP-1 antibody, sabe antibody, Mt1-mmp antibody, MT-MMPbeta antibody, cb386 antibody, sb:cb386 antibody, wu:fb72b09 antibody, mmp14 antibody, matrix metallopeptidase 14 antibody, matrix metallopeptidase 14 (membrane-inserted) antibody, matrix metallopeptidase 14b (membrane-inserted) antibody, matrix metallopeptidase 14a (membrane-inserted) antibody, matrix metallopeptidase 14 L homeolog antibody, MMP14 antibody, mmp14 antibody, Mmp14 antibody, mmp14b antibody, mmp14a antibody, mmp14.L antibody
    背景
    Name/Gene ID: MMP14
    Subfamily: Metallopeptidase M10A
    Family: Protease

    Synonyms: MMP14, Membrane type-1 MMP, MT1-MMP, MT1MMP, MT-MMP, MT-MMP 1, Matrix metalloproteinase-14, MMP-14, MMP-X1, MTMMP1
    基因ID
    4323
    UniProt
    P50281
    途径
    Autophagy
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