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BRAF 抗体 (AA 754-765)

BRAF 适用: 人, 猴, 犬, 马 WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN205677
发货至: 中国
  • 抗原 See all BRAF 抗体
    BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
    抗原表位
    • 18
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    AA 754-765
    适用
    • 201
    • 72
    • 51
    • 5
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    • 3
    • 2
    • 1
    • 1
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    人, 猴, 犬, 马
    宿主
    • 183
    • 18
    • 2
    克隆类型
    • 167
    • 36
    多克隆
    标记
    • 122
    • 13
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    This BRAF antibody is un-conjugated
    应用范围
    • 114
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    Western Blotting (WB)
    特异性
    Recognizes full length recombinant human BRaf (plus 6His tag, Mr ~94kD) and B-Raf splice variants in cell lysates (Mr ~68-94kD). Species cross-reactivity: Rat and mouse.
    预测反应
    Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Horse, Opossum (100%) Mouse, Porcine, Turkey, Rabbit, Chicken, Platypus (92%) Bat (83%).
    纯化方法
    Protein A purified
    免疫原
    Synthetic peptide corresponding to amino acids 754-765 (C-IQAGGYGAFPVH) of human B-Raf, with an N-terminal cysteine added for conjugation to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Horse, Opossum (100%), Mouse, Porcine, Turkey, Rabbit, Chicken, Platypus (92%), Bat (83%).

    Type of Immunogen: Synthetic peptide - KLH conjugated
    亚型
    IgG
    Top Product
    Discover our top product BRAF Primary Antibody
  • 应用备注
    Approved: WB (1 - 2 μg/mL)

    Usage: Suitable for use in Western Blot, MAP Kinase Activation and MBP Phosphorylation assays. Western Blot: 1-2 μg/mL detects B-Raf splice variants in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme. MAP Kinase Activation and MBP Phosphorylation: 4 μg.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide 30 % glycerol.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeat freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原
    BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
    别名
    BRAF / B-Raf (BRAF 产品)
    别名
    B-RAF1 antibody, BRAF1 antibody, NS7 antibody, RAFB1 antibody, C-RMIL antibody, RMIL antibody, B-Raf antibody, braf1 antibody, rafb1 antibody, b-raf1 antibody, sierrabraf antibody, BRAF antibody, 9930012E13Rik antibody, AA120551 antibody, AA387315 antibody, AA473386 antibody, B-raf antibody, Braf-2 antibody, Braf2 antibody, C230098H17 antibody, C87398 antibody, D6Ertd631e antibody, braf antibody, fc48g01 antibody, wu:fc48g01 antibody, B-Raf proto-oncogene, serine/threonine kinase antibody, B-Raf proto-oncogene, serine/threonine kinase S homeolog antibody, Braf transforming gene antibody, si:dkey-222f8.3 antibody, BRAF antibody, Braf antibody, braf.S antibody, braf antibody, si:dkey-222f8.3 antibody
    背景
    Name/Gene ID: BRAF
    Subfamily: Raf
    Family: Protein Kinase

    Synonyms: BRAF, 94 kDa B-raf protein, B-raf, BRAF1, B Raf, Proto-oncogene B-Raf, p94, B-RAF1, NS7, ONCOGENE BRAF, RAFB1
    基因ID
    673
    UniProt
    P15056
    途径
    MAPK Pathway, RTK signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy
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