BRAF 抗体 (AA 754-765)
Our Local Distributor
北京 101111
Quick Overview for BRAF 抗体 (AA 754-765) (ABIN205677)
抗原
See all BRAF 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 754-765
-
特异性
- Recognizes full length recombinant human BRaf (plus 6His tag, Mr ~94kD) and B-Raf splice variants in cell lysates (Mr ~68-94kD). Species cross-reactivity: Rat and mouse.
-
预测反应
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Horse, Opossum (100%) Mouse, Porcine, Turkey, Rabbit, Chicken, Platypus (92%) Bat (83%).
-
纯化方法
- Protein A purified
-
免疫原
-
Synthetic peptide corresponding to amino acids 754-765 (C-IQAGGYGAFPVH) of human B-Raf, with an N-terminal cysteine added for conjugation to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Horse, Opossum (100%), Mouse, Porcine, Turkey, Rabbit, Chicken, Platypus (92%), Bat (83%).
Type of Immunogen: Synthetic peptide - KLH conjugated -
亚型
- IgG
-
-
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibody
BRAF 适用: 人, 大鼠, 猴, 犬 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3A7 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 5A9 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人, 犬 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4C11 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 346-445) antibodyBRAF 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3D2 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 1H12F1 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 424-453) antibodyBRAF 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人, 小鼠 WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 1H12 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人, 小鼠 WB, IF 宿主: 小鼠 Monoclonal 5C5 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Approved: WB (1 - 2 μg/mL)
Usage: Suitable for use in Western Blot, MAP Kinase Activation and MBP Phosphorylation assays. Western Blot: 1-2 μg/mL detects B-Raf splice variants in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme. MAP Kinase Activation and MBP Phosphorylation: 4 μg. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide 30 % glycerol.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeat freeze-thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
-
- BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
-
别名
- BRAF / B-Raf
-
背景
-
Name/Gene ID: BRAF
Subfamily: Raf
Family: Protein Kinase
Synonyms: BRAF, 94 kDa B-raf protein, B-raf, BRAF1, B Raf, Proto-oncogene B-Raf, p94, B-RAF1, NS7, ONCOGENE BRAF, RAFB1 -
基因ID
- 673
-
UniProt
- P15056
-
途径
- MAPK Pathway, RTK signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy
抗原
-
