MEK1 抗体 (pSer218, pSer221)
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Quick Overview for MEK1 抗体 (pSer218, pSer221) (ABIN196944)
抗原
See all MEK1 (MAP2K1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer218, pSer221
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特异性
- This antibody detects endogenous levels of MEK1/MEK2 only when phosphorylated at Serine 217/221.
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纯化方法
- Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- Peptide sequence around the phosphorylation site of Serine 217/221 (I-D-Sp-M-A) derived from Human MEK1/MEK2
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anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody
MAP2K1 适用: 人 ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyMAP2K1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3H3 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyMAP2K1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 2H3 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 1-100) antibodyMAP2K1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer298) antibodyKD Validated MAP2K1 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB285 unconjugated Recombinant Antibody
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyMAP2K1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3B4 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyKD Validated MAP2K1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB2475 unconjugated Recombinant Antibody
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (N-Term) antibodyMAP2K1 适用: 人, 小鼠, 大鼠, 非洲爪蟾 WB, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer221) antibodyMAP2K1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr292) antibodyKD Validated MAP2K1 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB305 unconjugated Recombinant Antibody
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应用备注
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Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, containing 150 mM NaCl and 50 % Glycerol, 0.02 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at -20 °C.
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- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
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别名
- MAP2K1
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背景
- Synonyms: Dual specificity mitogen-activated protein kinase kinase 1/2, ERK activator kinase 1, ERK activator kinase 2, MAP kinase kinase 1, MAP kinase kinase 2, MAPK/ERK kinase 1, MAPK/ERK kinase 2, MAPKK1, MEK1, MEK2, PRKMK1, PRKMK2
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分子量
- 45 kDa
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基因ID
- 5604
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NCBI登录号
- NP_002746
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UniProt
- Q02750
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途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
抗原
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