ATF2 抗体 (pThr53, pThr71)
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Quick Overview for ATF2 抗体 (pThr53, pThr71) (ABIN196666)
抗原
See all ATF2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr53, pThr71
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特异性
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. ATF-2 (phospho-Thr71 or 53) antibody detects endogenous levels of ATF-2 only when phosphorylated at threonine 71 or 53.
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纯化方法
- Immunoaffinity chromatography.
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免疫原
- The antiserum was produced against synthesized phosphopeptide derived from human ATF-2 around the phosphorylation site of threonine 71 or 53 (T-P-TP-P-T).
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anti-Activating Transcription Factor 2 (ATF2) (AA 1-190) antibody
ATF2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 2 (ATF2) (pThr51), (pThr69) antibodyATF2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 2 (ATF2) (pThr53), (pThr71) antibodyATF2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 2 (ATF2) (pSer44), (pSer62) antibodyATF2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 2 (ATF2) (AA 93-450) antibodyATF2 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (fro), FACS 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 2 (ATF2) (C-Term) antibodyATF2 适用: 人, 小鼠, 大鼠, Cow, 兔, 犬, 马, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 2 (ATF2) (pThr55), (pThr73) antibodyATF2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 2 (ATF2) (pSer112), (pSer94) antibodyATF2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 2 (ATF2) (AA 91-190) antibodyATF2 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Activating Transcription Factor 2 (ATF2) (AA 1-209) antibodyATF2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1E12 unconjugated
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应用备注
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Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at -20 °C.
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- ATF2 (Activating Transcription Factor 2 (ATF2))
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别名
- ATF2
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背景
- ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.Synonyms: ATF-2, Activating transcription factor 2, CRE-BP1, CREB-2, CREB2, CREBP1, Cyclic AMP-dependent transcription factor ATF-2, Cyclic AMP-responsive element-binding protein 2, HB16, cAMP response element-binding protein CRE-BP1, cAMP-dependent transcription factor ATF-2
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基因ID
- 1386
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NCBI登录号
- NP_001871
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UniProt
- P15336
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途径
- MAPK Pathway, RTK signaling, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades
抗原
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