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Retinoblastoma 1 抗体 (pSer249, pTyr252)

Name: Retinoblastoma 1 抗体 (pSer249, pTyr252)
SKU: ABIN189976
Availability: OutOfStock

This anti-Retinoblastoma 1 antibody is a 兔多克隆 antibody detecting Retinoblastoma 1 in WB. Suitable for 人.

产品编号 ABIN189976

发货至: 中国

Quick Overview for Retinoblastoma 1 抗体 (pSer249, pTyr252) (ABIN189976)

抗原

See all Retinoblastoma 1 (RB1) 抗体

Retinoblastoma 1 (RB1)

适用

人

宿主

兔

克隆类型

多克隆

标记

This Retinoblastoma 1 antibody is un-conjugated

应用范围

Western Blotting (WB)

Discover our top product RB1 Primary Antibody

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pSer249, pTyr252

原理

Anti-Retinoblastoma phosphoserine 249/phosphothreonine 252 Rabbit Polyclonal Antibody

特异性

This antibody recognizes human Rb protein phosphorylated at serine 249 and threonine 252. Reactivity with other species has not been investigated.

交叉反应

人

纯化方法

Affinity - Purified

免疫原

Phosphopeptide region of human Rb protein containing Serine 249 and threonine 252.
anti-Retinoblastoma 1 (RB1) (pSer780) antibody
RB1 适用: 人, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer795) antibody
RB1 适用: 人 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer780) antibody
RB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (C-Term) antibody
RB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer807) antibody
RB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer795) antibody
RB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer780) antibody
RB1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated

anti-Retinoblastoma 1 (RB1) (C-Term) antibody
RB1 适用: 人 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer807), (pSer811) antibody
RB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (AA 639-778) antibody
RB1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Immunoblotting: use at 0.25-1 μg/mL.
End users should determine optimal concentration for their application.

限制

仅限研究用
状态

Liquid

溶解方式

Dilute in PBS or medium which is identical to that used in the assay system.

浓度

1.0 mg/mL

缓冲液

PBS, pH 7.4, 0.05 % sodium azide, 1 mg/mL BSA.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-80 °C

储存方法

Store at -80°C. Prepare appropriate aliquots to avoid multiple freeze-thaw cycles.
抗原

Retinoblastoma 1 (RB1)

别名

Retinoblastoma-Associated Protein

背景

Retinoblastoma-associated protein,Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). {UniProtKB:P13405, UniProtKB:P33568, PubMed:10499802, PubMed:15084261}., (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. {PubMed:1316611, PubMed:17974914, PubMed:18701596, PubMed:2839300, PubMed:8892909}.,Nucleus,p105-Rb, p110-RB1, pRb, Rb, pp110

基因ID

5925

NCBI登录号

NP_000312

UniProt

P06400

途径

Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy