GADD34 抗体 (C-Term)
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- 抗原 See all GADD34 (PPP1R15A) 抗体
- GADD34 (PPP1R15A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This GADD34 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- 原理
- PPP1R15A / GADD34
- 序列
- AAALDLSGRR G
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence C-AAALDLSGRRG, from the C Terminus of the protein sequence according to NP_055145.3.
- 亚型
- IgG
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anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) antibody
PPP1R15A 适用: 人, 小鼠, 大鼠, 猴, 犬 WB, IHC, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal 3D12 unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) (AA 375-674) antibodyPPP1R15A 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) (AA 401-500) antibodyPPP1R15A 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) (AA 401-500) antibody (Alexa Fluor 647)PPP1R15A 适用: 人, 小鼠, 大鼠 WB, FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal Alexa Fluor 647
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) (AA 430-490) antibodyPPP1R15A 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) antibodyPPP1R15A 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) (AA 116-165) antibodyPPP1R15A 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) (AA 433-636), (Regulatory Subunit 15A) antibodyPPP1R15A 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) (AA 335-578), (Regulatory Subunit 15A) antibodyPPP1R15A 适用: 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry: In paraffin embedded Human Liver shows cytoplasmic staining consistent with ER in the hepatocytes. Recommended concentration: 2-4 μg/mL.
Western Blot: Approx 75 kDa band observed in lysates of cell line HepG2 (calculated MW of 73.4 kDa according to NP_055145.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature. Negative Control: KLY (Kel
Peptide ELISA: antibody detection limit dilution 1:128000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and HepG2 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration:
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- GADD34 (PPP1R15A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A))
- 别名
- PPP1R15A (PPP1R15A 产品)
- 别名
- 9630030H21 antibody, Gadd34 antibody, Myd116 antibody, GADD34 antibody, Peg-3 antibody, RGD1624209 antibody, protein phosphatase 1 regulatory subunit 15A antibody, protein phosphatase 1, regulatory (inhibitor) subunit 15A antibody, protein phosphatase 1, regulatory subunit 15A antibody, PPP1R15A antibody, Ppp1r15a antibody
- 背景
- PPP1R15A, GADD34, protein phosphatase 1, regulatory (inhibitor) subunit 15A, growth arrest and DNA-damage-inducible 34, protein phosphatase 1, regulatory subunit 15A
- 基因ID
- 23645
- NCBI登录号
- NP_055145
- 途径
- ER-Nucleus Signaling, Unfolded Protein Response
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