ELISA: Indirect: To detect hWNT-3a by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a. Sandwich: To detect hWNT-3a by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with Biotinylated Anti-HumanWNT-3a as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hWNT-3a. Western blot: 0.1 - 0.2 μg/mL (reducing and non-reducing conditions). Neutralisation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
缓冲液
PBS, pH 7.2
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
有效期
6 months
抗原
WNT3A
(Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))