Abeta 38/40/42 (AA 1-16), (AA 4-16) 抗体
(2 references)-
- 抗原
- Abeta 38/40/42
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抗原表位
- AA 1-16, AA 4-16
- 适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Immunohistochemistry (IHC), Western Blotting (WB), ELISA
- 特异性
- Specific for Abeta 38, 40, 42.
- 纯化方法
- purified IgG. Azide was added before lyophilization.
- 免疫原
- Synthetic peptide (aa 1-16) of abeta peptides 38, 40, 42 coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue. Epitope aa 4-16
- 克隆位点
- NT78
- 亚型
- IgG2b
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anti-Abeta 38/40/42 (AA 1-16), (AA 4-8) antibody
适用: 人, 小鼠 IHC, WB 宿主: 小鼠 Monoclonal NT244 unconjugated
anti-Abeta 38/40/42 (AA 1-16), (AA 4-16) antibody (Biotin)适用: 人, 小鼠 IHC, WB, ELISA 宿主: 小鼠 Monoclonal NT78 Biotin
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- 应用备注
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WB: 1 : 500 up to 1 : 1000
IP: not tested yet
ICC: not tested yet
IHC-P: 1 : 200 up to 1 : 500 - 说明
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ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- 缓冲液
- PBS, 0.02% sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
- 储存条件
- -20 °C
- 储存方法
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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: "Overexpression of glutaminyl cyclase, the enzyme responsible for pyroglutamate A{beta} formation, induces behavioral deficits, and glutaminyl cyclase knock-out rescues the behavioral phenotype in ..." in: The Journal of biological chemistry, Vol. 286, Issue 6, pp. 4454-60, (2011) (PubMed).
: "Identification of low molecular weight pyroglutamate A{beta} oligomers in Alzheimer disease: a novel tool for therapy and diagnosis." in: The Journal of biological chemistry, Vol. 285, Issue 53, pp. 41517-24, (2010) (PubMed).
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: "Overexpression of glutaminyl cyclase, the enzyme responsible for pyroglutamate A{beta} formation, induces behavioral deficits, and glutaminyl cyclase knock-out rescues the behavioral phenotype in ..." in: The Journal of biological chemistry, Vol. 286, Issue 6, pp. 4454-60, (2011) (PubMed).
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- 抗原
- Abeta 38/40/42
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